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STEMCELL Technologies Inc cd34+ human cord blood stem cells
In vitro characterisation of FLT3L-Fc NG2LH activity . Representative sensorgrams of FLT3L-Fc NG2LH binding to recombinant human monomeric FLT3-monoFc (a) and dimeric FLT3-Fc (b). Black solid lines are curves fit to the data using a 1:1 binding model, and coloured lines are raw data. The tested recombinant human FLT3-monoFc concentrations (from bottom to top) are 62.5, 125, 250, 500, 1000, and 2000 nM; recombinant human dimeric FLT3-Fc concentrations (from bottom to top) are 3.75, 7.5, 15, 30, 60, and 120 nM. The sensorgrams were generated after in-line reference cell correction followed by buffer sample subtraction. The experiments were conducted at 37 °C. (c) Binding affinities of recombinant human monomeric FLT3-monoFc and dimeric FLT3-Fc proteins to captured FLT3L-Fc NG2LH. k a association rate constant; k d dissociation rate constant; K D equilibrium dissociation constant. Data for FLT3L-Fc NG2LH binding to recombinant human FLT3-monoFc were averaged from three independent experimental Biacore runs. Data for FLT3L-Fc NG2LH binding to recombinant human dimeric FLT3-Fc were averaged from two independent experimental Biacore runs. (d) ADCP for FLT3L-Fc NG2LH and its variants. Data shown are a representative set taken from one of three independent experiments. Average (SD) of duplicate values are shown. (e) Proliferation of OCI-AML5 cells in vitro. A representative dose response curve is shown, Average (SD) of triplicate values. Data were normalised to 10 μg/mL FLT3L-Fc NG2LH as the maximum (100%) response and the assay media alone control as the minimum (0%) response. (f) Human cDC1 differentiation from cord blood <t>stem</t> <t>cells.</t> Frequency of cDC1 at the end of culture is shown (percent of live cells). Results were obtained from 2 donors. (g) In vitro proliferation of mouse bone marrow cells in response to mFLT3L or mFLT3L-Fc. Average (SD) of duplicate values are shown.
Cd34+ Human Cord Blood Stem Cells, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Article Title: Effectorless Fc-fusion improves FLT3L drug-like properties for cancer immunotherapy combinations

Journal: eBioMedicine

doi: 10.1016/j.ebiom.2025.105822

In vitro characterisation of FLT3L-Fc NG2LH activity . Representative sensorgrams of FLT3L-Fc NG2LH binding to recombinant human monomeric FLT3-monoFc (a) and dimeric FLT3-Fc (b). Black solid lines are curves fit to the data using a 1:1 binding model, and coloured lines are raw data. The tested recombinant human FLT3-monoFc concentrations (from bottom to top) are 62.5, 125, 250, 500, 1000, and 2000 nM; recombinant human dimeric FLT3-Fc concentrations (from bottom to top) are 3.75, 7.5, 15, 30, 60, and 120 nM. The sensorgrams were generated after in-line reference cell correction followed by buffer sample subtraction. The experiments were conducted at 37 °C. (c) Binding affinities of recombinant human monomeric FLT3-monoFc and dimeric FLT3-Fc proteins to captured FLT3L-Fc NG2LH. k a association rate constant; k d dissociation rate constant; K D equilibrium dissociation constant. Data for FLT3L-Fc NG2LH binding to recombinant human FLT3-monoFc were averaged from three independent experimental Biacore runs. Data for FLT3L-Fc NG2LH binding to recombinant human dimeric FLT3-Fc were averaged from two independent experimental Biacore runs. (d) ADCP for FLT3L-Fc NG2LH and its variants. Data shown are a representative set taken from one of three independent experiments. Average (SD) of duplicate values are shown. (e) Proliferation of OCI-AML5 cells in vitro. A representative dose response curve is shown, Average (SD) of triplicate values. Data were normalised to 10 μg/mL FLT3L-Fc NG2LH as the maximum (100%) response and the assay media alone control as the minimum (0%) response. (f) Human cDC1 differentiation from cord blood stem cells. Frequency of cDC1 at the end of culture is shown (percent of live cells). Results were obtained from 2 donors. (g) In vitro proliferation of mouse bone marrow cells in response to mFLT3L or mFLT3L-Fc. Average (SD) of duplicate values are shown.
Figure Legend Snippet: In vitro characterisation of FLT3L-Fc NG2LH activity . Representative sensorgrams of FLT3L-Fc NG2LH binding to recombinant human monomeric FLT3-monoFc (a) and dimeric FLT3-Fc (b). Black solid lines are curves fit to the data using a 1:1 binding model, and coloured lines are raw data. The tested recombinant human FLT3-monoFc concentrations (from bottom to top) are 62.5, 125, 250, 500, 1000, and 2000 nM; recombinant human dimeric FLT3-Fc concentrations (from bottom to top) are 3.75, 7.5, 15, 30, 60, and 120 nM. The sensorgrams were generated after in-line reference cell correction followed by buffer sample subtraction. The experiments were conducted at 37 °C. (c) Binding affinities of recombinant human monomeric FLT3-monoFc and dimeric FLT3-Fc proteins to captured FLT3L-Fc NG2LH. k a association rate constant; k d dissociation rate constant; K D equilibrium dissociation constant. Data for FLT3L-Fc NG2LH binding to recombinant human FLT3-monoFc were averaged from three independent experimental Biacore runs. Data for FLT3L-Fc NG2LH binding to recombinant human dimeric FLT3-Fc were averaged from two independent experimental Biacore runs. (d) ADCP for FLT3L-Fc NG2LH and its variants. Data shown are a representative set taken from one of three independent experiments. Average (SD) of duplicate values are shown. (e) Proliferation of OCI-AML5 cells in vitro. A representative dose response curve is shown, Average (SD) of triplicate values. Data were normalised to 10 μg/mL FLT3L-Fc NG2LH as the maximum (100%) response and the assay media alone control as the minimum (0%) response. (f) Human cDC1 differentiation from cord blood stem cells. Frequency of cDC1 at the end of culture is shown (percent of live cells). Results were obtained from 2 donors. (g) In vitro proliferation of mouse bone marrow cells in response to mFLT3L or mFLT3L-Fc. Average (SD) of duplicate values are shown.

Techniques Used: In Vitro, Activity Assay, Binding Assay, Recombinant, Generated, Control

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In Vitro:

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Activity Assay:

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Binding Assay:

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Article Title: Lactate dehydrogenase A-coupled NAD + regeneration is critical for acute myeloid leukemia cell survival
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Article Title: Perturbing LSD1 and WNT rewires transcription to synergistically induce AML differentiation.
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Article Snippet: CD34+ human cord blood-derived cells were obtained from STEMCELL Technologies and were maintained in StemSpan SFEM-II with CD34 supplements (STEMCELL Technologies).

Recombinant:

Article Title: Effectorless Fc-fusion improves FLT3L drug-like properties for cancer immunotherapy combinations
Article Snippet: CD34+ human cord blood stem cells (StemCell Technologies) were seeded at 5000 cells per well in 96-well U-bottom plates (Costar) in 100 μL expansion media (StemSpan media–StemCell Technologies, 10% FBS, 40 ng/mL IL-3, 200 ng/mL SCF, 100 ng/mL TPO–cytokines from Peprotech).

Article Title: Perturbing LSD1 and WNT rewires transcription to synergistically induce AML differentiation
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Article Title: Utility of CK8/18 in identifying circulating tumor cells derived from lesions in patients with non-small cell lung cancer
Article Snippet: Human stem cells (Human Cord Blood CD34 + cell) were obtained from Stem Cell Technologies (Vancouver, Canada).

Article Title: Quantification of intrinsic regulatory factors refines human hematopoietic progenitor definitions and reveals early erythroid lineage priming.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies All antibodies used for cytometry Various Table S1 Biological samples Human BM CD34+ cells bluebird bio n/a Human mPB CD34+ cells bluebird bio n/a Human MNC from FL AllCells n/a Cord Blood CD34+ cells STEMCELL Technologies n/a Chemicals, peptides, and recombinant proteins Ficoll-Paque Plus GE Healthcare Cat#300-25 Bovine Serum Album (BSA) Heat-shock Treated Thermo Fisher Scientific Cat#BP1600-100 Human TruStain FcX (Fc Receptor Blocking Solution) BioLegend Cat#422302 Cell-ID Intercalator-Ir Standard BioTools Inc. Cat#201192A Cell-ID Cisplatin Standard BioTools Inc. Cat#201064 Saponin from quillaja bark Sigma-Aldrich Cat#S7900-25G Calibration Beads, EQ, Four Element Standard BioTools Inc. Cat#201078 7-AAD Viability Staining Solution BioLegend Cat#420404 Benzonase nuclease Sigma-Aldrich Cat#E1014-25KU Deposited data Mass cytometry and integration data and code This study https://doi.org/10.5281/zenodo.13968159 ATAC-seq processed data and code This study https://doi.org/10.5281/zenodo.8266662 ATAC-seq sequence data This study GEO: GSE241229 Experimental models: Organisms/strains NSG mice (NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ) Jackson Laboratory N/A Recombinant DNA EGFP-VSVg-ProtC-AU1 Addgene N/A mCherry-StrepTagII-FLAG-AU1 Addgene N/A Software and algorithms RStudio RStudio rstudio.com Cytobank BeckmanCoulter beckman.com CellEngine Primity Bio cellengine.com MATLAB MathWorks mathworks.co Cell Reports 44, 115913, July 22, 2025 19

Article Title: Effectorless Fc-fusion improves FLT3L drug-like properties for cancer immunotherapy combinations.
Article Snippet: 41 CD34+ human cord blood stem cells (StemCell Technologies) were seeded at 5000 cells per well in 96-well U-bottom plates (Costar) in 100 μL expansion media (StemSpan media–StemCell Technologies, 10% FBS, 40 ng/mL IL-3, 200 ng/mL SCF, 100 ng/mL TPO–cytokines from Peprotech).

Article Title: Lactate dehydrogenase A-coupled NAD + regeneration is critical for acute myeloid leukemia cell survival
Article Snippet: CD34 + human cord blood-derived cells were obtained from STEMCELL Technologies and were maintained in StemSpan SFEM-II with CD34 supplements (STEMCELL Technologies).

Article Title: Perturbing LSD1 and WNT rewires transcription to synergistically induce AML differentiation.
Article Snippet: Human cord blood CD34+ cells (200-0000, StemCell Technologies) were plated in methylcellulose (MethoCult H4534 Classic, StemCell Technologies).

Article Title: Lactate dehydrogenase A-coupled NAD + regeneration is critical for acute myeloid leukemia cell survival.
Article Snippet: CD34+ human cord blood-derived cells were obtained from STEMCELL Technologies and were maintained in StemSpan SFEM-II with CD34 supplements (STEMCELL Technologies).

Generated:

Article Title: Effectorless Fc-fusion improves FLT3L drug-like properties for cancer immunotherapy combinations
Article Snippet: CD34+ human cord blood stem cells (StemCell Technologies) were seeded at 5000 cells per well in 96-well U-bottom plates (Costar) in 100 μL expansion media (StemSpan media–StemCell Technologies, 10% FBS, 40 ng/mL IL-3, 200 ng/mL SCF, 100 ng/mL TPO–cytokines from Peprotech).

Article Title: Perturbing LSD1 and WNT rewires transcription to synergistically induce AML differentiation
Article Snippet: Human cord blood CD34 + cells (200-0000, StemCell Technologies) were plated in methylcellulose (MethoCult H4534 Classic, StemCell Technologies).

Article Title: Utility of CK8/18 in identifying circulating tumor cells derived from lesions in patients with non-small cell lung cancer
Article Snippet: Human stem cells (Human Cord Blood CD34 + cell) were obtained from Stem Cell Technologies (Vancouver, Canada).

Article Title: Quantification of intrinsic regulatory factors refines human hematopoietic progenitor definitions and reveals early erythroid lineage priming.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies All antibodies used for cytometry Various Table S1 Biological samples Human BM CD34+ cells bluebird bio n/a Human mPB CD34+ cells bluebird bio n/a Human MNC from FL AllCells n/a Cord Blood CD34+ cells STEMCELL Technologies n/a Chemicals, peptides, and recombinant proteins Ficoll-Paque Plus GE Healthcare Cat#300-25 Bovine Serum Album (BSA) Heat-shock Treated Thermo Fisher Scientific Cat#BP1600-100 Human TruStain FcX (Fc Receptor Blocking Solution) BioLegend Cat#422302 Cell-ID Intercalator-Ir Standard BioTools Inc. Cat#201192A Cell-ID Cisplatin Standard BioTools Inc. Cat#201064 Saponin from quillaja bark Sigma-Aldrich Cat#S7900-25G Calibration Beads, EQ, Four Element Standard BioTools Inc. Cat#201078 7-AAD Viability Staining Solution BioLegend Cat#420404 Benzonase nuclease Sigma-Aldrich Cat#E1014-25KU Deposited data Mass cytometry and integration data and code This study https://doi.org/10.5281/zenodo.13968159 ATAC-seq processed data and code This study https://doi.org/10.5281/zenodo.8266662 ATAC-seq sequence data This study GEO: GSE241229 Experimental models: Organisms/strains NSG mice (NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ) Jackson Laboratory N/A Recombinant DNA EGFP-VSVg-ProtC-AU1 Addgene N/A mCherry-StrepTagII-FLAG-AU1 Addgene N/A Software and algorithms RStudio RStudio rstudio.com Cytobank BeckmanCoulter beckman.com CellEngine Primity Bio cellengine.com MATLAB MathWorks mathworks.co Cell Reports 44, 115913, July 22, 2025 19

Article Title: Effectorless Fc-fusion improves FLT3L drug-like properties for cancer immunotherapy combinations.
Article Snippet: 41 CD34+ human cord blood stem cells (StemCell Technologies) were seeded at 5000 cells per well in 96-well U-bottom plates (Costar) in 100 μL expansion media (StemSpan media–StemCell Technologies, 10% FBS, 40 ng/mL IL-3, 200 ng/mL SCF, 100 ng/mL TPO–cytokines from Peprotech).

Article Title: Lactate dehydrogenase A-coupled NAD + regeneration is critical for acute myeloid leukemia cell survival
Article Snippet: CD34 + human cord blood-derived cells were obtained from STEMCELL Technologies and were maintained in StemSpan SFEM-II with CD34 supplements (STEMCELL Technologies).

Article Title: Perturbing LSD1 and WNT rewires transcription to synergistically induce AML differentiation.
Article Snippet: Human cord blood CD34+ cells (200-0000, StemCell Technologies) were plated in methylcellulose (MethoCult H4534 Classic, StemCell Technologies).

Article Title: Lactate dehydrogenase A-coupled NAD + regeneration is critical for acute myeloid leukemia cell survival.
Article Snippet: CD34+ human cord blood-derived cells were obtained from STEMCELL Technologies and were maintained in StemSpan SFEM-II with CD34 supplements (STEMCELL Technologies).

Control:

Article Title: Effectorless Fc-fusion improves FLT3L drug-like properties for cancer immunotherapy combinations
Article Snippet: CD34+ human cord blood stem cells (StemCell Technologies) were seeded at 5000 cells per well in 96-well U-bottom plates (Costar) in 100 μL expansion media (StemSpan media–StemCell Technologies, 10% FBS, 40 ng/mL IL-3, 200 ng/mL SCF, 100 ng/mL TPO–cytokines from Peprotech).

Article Title: Perturbing LSD1 and WNT rewires transcription to synergistically induce AML differentiation
Article Snippet: Human cord blood CD34 + cells (200-0000, StemCell Technologies) were plated in methylcellulose (MethoCult H4534 Classic, StemCell Technologies).

Article Title: Utility of CK8/18 in identifying circulating tumor cells derived from lesions in patients with non-small cell lung cancer
Article Snippet: Human stem cells (Human Cord Blood CD34 + cell) were obtained from Stem Cell Technologies (Vancouver, Canada).

Article Title: Quantification of intrinsic regulatory factors refines human hematopoietic progenitor definitions and reveals early erythroid lineage priming.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies All antibodies used for cytometry Various Table S1 Biological samples Human BM CD34+ cells bluebird bio n/a Human mPB CD34+ cells bluebird bio n/a Human MNC from FL AllCells n/a Cord Blood CD34+ cells STEMCELL Technologies n/a Chemicals, peptides, and recombinant proteins Ficoll-Paque Plus GE Healthcare Cat#300-25 Bovine Serum Album (BSA) Heat-shock Treated Thermo Fisher Scientific Cat#BP1600-100 Human TruStain FcX (Fc Receptor Blocking Solution) BioLegend Cat#422302 Cell-ID Intercalator-Ir Standard BioTools Inc. Cat#201192A Cell-ID Cisplatin Standard BioTools Inc. Cat#201064 Saponin from quillaja bark Sigma-Aldrich Cat#S7900-25G Calibration Beads, EQ, Four Element Standard BioTools Inc. Cat#201078 7-AAD Viability Staining Solution BioLegend Cat#420404 Benzonase nuclease Sigma-Aldrich Cat#E1014-25KU Deposited data Mass cytometry and integration data and code This study https://doi.org/10.5281/zenodo.13968159 ATAC-seq processed data and code This study https://doi.org/10.5281/zenodo.8266662 ATAC-seq sequence data This study GEO: GSE241229 Experimental models: Organisms/strains NSG mice (NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ) Jackson Laboratory N/A Recombinant DNA EGFP-VSVg-ProtC-AU1 Addgene N/A mCherry-StrepTagII-FLAG-AU1 Addgene N/A Software and algorithms RStudio RStudio rstudio.com Cytobank BeckmanCoulter beckman.com CellEngine Primity Bio cellengine.com MATLAB MathWorks mathworks.co Cell Reports 44, 115913, July 22, 2025 19

Article Title: Effectorless Fc-fusion improves FLT3L drug-like properties for cancer immunotherapy combinations.
Article Snippet: 41 CD34+ human cord blood stem cells (StemCell Technologies) were seeded at 5000 cells per well in 96-well U-bottom plates (Costar) in 100 μL expansion media (StemSpan media–StemCell Technologies, 10% FBS, 40 ng/mL IL-3, 200 ng/mL SCF, 100 ng/mL TPO–cytokines from Peprotech).

Article Title: Lactate dehydrogenase A-coupled NAD + regeneration is critical for acute myeloid leukemia cell survival
Article Snippet: CD34 + human cord blood-derived cells were obtained from STEMCELL Technologies and were maintained in StemSpan SFEM-II with CD34 supplements (STEMCELL Technologies).

Article Title: Perturbing LSD1 and WNT rewires transcription to synergistically induce AML differentiation.
Article Snippet: Human cord blood CD34+ cells (200-0000, StemCell Technologies) were plated in methylcellulose (MethoCult H4534 Classic, StemCell Technologies).

Article Title: Lactate dehydrogenase A-coupled NAD + regeneration is critical for acute myeloid leukemia cell survival.
Article Snippet: CD34+ human cord blood-derived cells were obtained from STEMCELL Technologies and were maintained in StemSpan SFEM-II with CD34 supplements (STEMCELL Technologies).

Cytometry:

Article Title: Effectorless Fc-fusion improves FLT3L drug-like properties for cancer immunotherapy combinations
Article Snippet: CD34+ human cord blood stem cells (StemCell Technologies) were seeded at 5000 cells per well in 96-well U-bottom plates (Costar) in 100 μL expansion media (StemSpan media–StemCell Technologies, 10% FBS, 40 ng/mL IL-3, 200 ng/mL SCF, 100 ng/mL TPO–cytokines from Peprotech).

Article Title: Perturbing LSD1 and WNT rewires transcription to synergistically induce AML differentiation
Article Snippet: Human cord blood CD34 + cells (200-0000, StemCell Technologies) were plated in methylcellulose (MethoCult H4534 Classic, StemCell Technologies).

Article Title: Utility of CK8/18 in identifying circulating tumor cells derived from lesions in patients with non-small cell lung cancer
Article Snippet: Human stem cells (Human Cord Blood CD34 + cell) were obtained from Stem Cell Technologies (Vancouver, Canada).

Article Title: Quantification of intrinsic regulatory factors refines human hematopoietic progenitor definitions and reveals early erythroid lineage priming.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies All antibodies used for cytometry Various Table S1 Biological samples Human BM CD34+ cells bluebird bio n/a Human mPB CD34+ cells bluebird bio n/a Human MNC from FL AllCells n/a Cord Blood CD34+ cells STEMCELL Technologies n/a Chemicals, peptides, and recombinant proteins Ficoll-Paque Plus GE Healthcare Cat#300-25 Bovine Serum Album (BSA) Heat-shock Treated Thermo Fisher Scientific Cat#BP1600-100 Human TruStain FcX (Fc Receptor Blocking Solution) BioLegend Cat#422302 Cell-ID Intercalator-Ir Standard BioTools Inc. Cat#201192A Cell-ID Cisplatin Standard BioTools Inc. Cat#201064 Saponin from quillaja bark Sigma-Aldrich Cat#S7900-25G Calibration Beads, EQ, Four Element Standard BioTools Inc. Cat#201078 7-AAD Viability Staining Solution BioLegend Cat#420404 Benzonase nuclease Sigma-Aldrich Cat#E1014-25KU Deposited data Mass cytometry and integration data and code This study https://doi.org/10.5281/zenodo.13968159 ATAC-seq processed data and code This study https://doi.org/10.5281/zenodo.8266662 ATAC-seq sequence data This study GEO: GSE241229 Experimental models: Organisms/strains NSG mice (NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ) Jackson Laboratory N/A Recombinant DNA EGFP-VSVg-ProtC-AU1 Addgene N/A mCherry-StrepTagII-FLAG-AU1 Addgene N/A Software and algorithms RStudio RStudio rstudio.com Cytobank BeckmanCoulter beckman.com CellEngine Primity Bio cellengine.com MATLAB MathWorks mathworks.co Cell Reports 44, 115913, July 22, 2025 19

Article Title: Effectorless Fc-fusion improves FLT3L drug-like properties for cancer immunotherapy combinations.
Article Snippet: 41 CD34+ human cord blood stem cells (StemCell Technologies) were seeded at 5000 cells per well in 96-well U-bottom plates (Costar) in 100 μL expansion media (StemSpan media–StemCell Technologies, 10% FBS, 40 ng/mL IL-3, 200 ng/mL SCF, 100 ng/mL TPO–cytokines from Peprotech).

Article Title: Lactate dehydrogenase A-coupled NAD + regeneration is critical for acute myeloid leukemia cell survival
Article Snippet: CD34 + human cord blood-derived cells were obtained from STEMCELL Technologies and were maintained in StemSpan SFEM-II with CD34 supplements (STEMCELL Technologies).

Article Title: Perturbing LSD1 and WNT rewires transcription to synergistically induce AML differentiation.
Article Snippet: Human cord blood CD34+ cells (200-0000, StemCell Technologies) were plated in methylcellulose (MethoCult H4534 Classic, StemCell Technologies).

Article Title: Lactate dehydrogenase A-coupled NAD + regeneration is critical for acute myeloid leukemia cell survival.
Article Snippet: CD34+ human cord blood-derived cells were obtained from STEMCELL Technologies and were maintained in StemSpan SFEM-II with CD34 supplements (STEMCELL Technologies).

Blocking Assay:

Article Title: Effectorless Fc-fusion improves FLT3L drug-like properties for cancer immunotherapy combinations
Article Snippet: CD34+ human cord blood stem cells (StemCell Technologies) were seeded at 5000 cells per well in 96-well U-bottom plates (Costar) in 100 μL expansion media (StemSpan media–StemCell Technologies, 10% FBS, 40 ng/mL IL-3, 200 ng/mL SCF, 100 ng/mL TPO–cytokines from Peprotech).

Article Title: Perturbing LSD1 and WNT rewires transcription to synergistically induce AML differentiation
Article Snippet: Human cord blood CD34 + cells (200-0000, StemCell Technologies) were plated in methylcellulose (MethoCult H4534 Classic, StemCell Technologies).

Article Title: Utility of CK8/18 in identifying circulating tumor cells derived from lesions in patients with non-small cell lung cancer
Article Snippet: Human stem cells (Human Cord Blood CD34 + cell) were obtained from Stem Cell Technologies (Vancouver, Canada).

Article Title: Quantification of intrinsic regulatory factors refines human hematopoietic progenitor definitions and reveals early erythroid lineage priming.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies All antibodies used for cytometry Various Table S1 Biological samples Human BM CD34+ cells bluebird bio n/a Human mPB CD34+ cells bluebird bio n/a Human MNC from FL AllCells n/a Cord Blood CD34+ cells STEMCELL Technologies n/a Chemicals, peptides, and recombinant proteins Ficoll-Paque Plus GE Healthcare Cat#300-25 Bovine Serum Album (BSA) Heat-shock Treated Thermo Fisher Scientific Cat#BP1600-100 Human TruStain FcX (Fc Receptor Blocking Solution) BioLegend Cat#422302 Cell-ID Intercalator-Ir Standard BioTools Inc. Cat#201192A Cell-ID Cisplatin Standard BioTools Inc. Cat#201064 Saponin from quillaja bark Sigma-Aldrich Cat#S7900-25G Calibration Beads, EQ, Four Element Standard BioTools Inc. Cat#201078 7-AAD Viability Staining Solution BioLegend Cat#420404 Benzonase nuclease Sigma-Aldrich Cat#E1014-25KU Deposited data Mass cytometry and integration data and code This study https://doi.org/10.5281/zenodo.13968159 ATAC-seq processed data and code This study https://doi.org/10.5281/zenodo.8266662 ATAC-seq sequence data This study GEO: GSE241229 Experimental models: Organisms/strains NSG mice (NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ) Jackson Laboratory N/A Recombinant DNA EGFP-VSVg-ProtC-AU1 Addgene N/A mCherry-StrepTagII-FLAG-AU1 Addgene N/A Software and algorithms RStudio RStudio rstudio.com Cytobank BeckmanCoulter beckman.com CellEngine Primity Bio cellengine.com MATLAB MathWorks mathworks.co Cell Reports 44, 115913, July 22, 2025 19

Article Title: Effectorless Fc-fusion improves FLT3L drug-like properties for cancer immunotherapy combinations.
Article Snippet: 41 CD34+ human cord blood stem cells (StemCell Technologies) were seeded at 5000 cells per well in 96-well U-bottom plates (Costar) in 100 μL expansion media (StemSpan media–StemCell Technologies, 10% FBS, 40 ng/mL IL-3, 200 ng/mL SCF, 100 ng/mL TPO–cytokines from Peprotech).

Article Title: Lactate dehydrogenase A-coupled NAD + regeneration is critical for acute myeloid leukemia cell survival
Article Snippet: CD34 + human cord blood-derived cells were obtained from STEMCELL Technologies and were maintained in StemSpan SFEM-II with CD34 supplements (STEMCELL Technologies).

Article Title: Perturbing LSD1 and WNT rewires transcription to synergistically induce AML differentiation.
Article Snippet: Human cord blood CD34+ cells (200-0000, StemCell Technologies) were plated in methylcellulose (MethoCult H4534 Classic, StemCell Technologies).

Article Title: Lactate dehydrogenase A-coupled NAD + regeneration is critical for acute myeloid leukemia cell survival.
Article Snippet: CD34+ human cord blood-derived cells were obtained from STEMCELL Technologies and were maintained in StemSpan SFEM-II with CD34 supplements (STEMCELL Technologies).

Staining:

Article Title: Effectorless Fc-fusion improves FLT3L drug-like properties for cancer immunotherapy combinations
Article Snippet: CD34+ human cord blood stem cells (StemCell Technologies) were seeded at 5000 cells per well in 96-well U-bottom plates (Costar) in 100 μL expansion media (StemSpan media–StemCell Technologies, 10% FBS, 40 ng/mL IL-3, 200 ng/mL SCF, 100 ng/mL TPO–cytokines from Peprotech).

Article Title: Perturbing LSD1 and WNT rewires transcription to synergistically induce AML differentiation
Article Snippet: Human cord blood CD34 + cells (200-0000, StemCell Technologies) were plated in methylcellulose (MethoCult H4534 Classic, StemCell Technologies).

Article Title: Utility of CK8/18 in identifying circulating tumor cells derived from lesions in patients with non-small cell lung cancer
Article Snippet: Human stem cells (Human Cord Blood CD34 + cell) were obtained from Stem Cell Technologies (Vancouver, Canada).

Article Title: Quantification of intrinsic regulatory factors refines human hematopoietic progenitor definitions and reveals early erythroid lineage priming.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies All antibodies used for cytometry Various Table S1 Biological samples Human BM CD34+ cells bluebird bio n/a Human mPB CD34+ cells bluebird bio n/a Human MNC from FL AllCells n/a Cord Blood CD34+ cells STEMCELL Technologies n/a Chemicals, peptides, and recombinant proteins Ficoll-Paque Plus GE Healthcare Cat#300-25 Bovine Serum Album (BSA) Heat-shock Treated Thermo Fisher Scientific Cat#BP1600-100 Human TruStain FcX (Fc Receptor Blocking Solution) BioLegend Cat#422302 Cell-ID Intercalator-Ir Standard BioTools Inc. Cat#201192A Cell-ID Cisplatin Standard BioTools Inc. Cat#201064 Saponin from quillaja bark Sigma-Aldrich Cat#S7900-25G Calibration Beads, EQ, Four Element Standard BioTools Inc. Cat#201078 7-AAD Viability Staining Solution BioLegend Cat#420404 Benzonase nuclease Sigma-Aldrich Cat#E1014-25KU Deposited data Mass cytometry and integration data and code This study https://doi.org/10.5281/zenodo.13968159 ATAC-seq processed data and code This study https://doi.org/10.5281/zenodo.8266662 ATAC-seq sequence data This study GEO: GSE241229 Experimental models: Organisms/strains NSG mice (NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ) Jackson Laboratory N/A Recombinant DNA EGFP-VSVg-ProtC-AU1 Addgene N/A mCherry-StrepTagII-FLAG-AU1 Addgene N/A Software and algorithms RStudio RStudio rstudio.com Cytobank BeckmanCoulter beckman.com CellEngine Primity Bio cellengine.com MATLAB MathWorks mathworks.co Cell Reports 44, 115913, July 22, 2025 19

Article Title: Effectorless Fc-fusion improves FLT3L drug-like properties for cancer immunotherapy combinations.
Article Snippet: 41 CD34+ human cord blood stem cells (StemCell Technologies) were seeded at 5000 cells per well in 96-well U-bottom plates (Costar) in 100 μL expansion media (StemSpan media–StemCell Technologies, 10% FBS, 40 ng/mL IL-3, 200 ng/mL SCF, 100 ng/mL TPO–cytokines from Peprotech).

Article Title: Lactate dehydrogenase A-coupled NAD + regeneration is critical for acute myeloid leukemia cell survival
Article Snippet: CD34 + human cord blood-derived cells were obtained from STEMCELL Technologies and were maintained in StemSpan SFEM-II with CD34 supplements (STEMCELL Technologies).

Article Title: Perturbing LSD1 and WNT rewires transcription to synergistically induce AML differentiation.
Article Snippet: Human cord blood CD34+ cells (200-0000, StemCell Technologies) were plated in methylcellulose (MethoCult H4534 Classic, StemCell Technologies).

Article Title: Lactate dehydrogenase A-coupled NAD + regeneration is critical for acute myeloid leukemia cell survival.
Article Snippet: CD34+ human cord blood-derived cells were obtained from STEMCELL Technologies and were maintained in StemSpan SFEM-II with CD34 supplements (STEMCELL Technologies).

Mass Cytometry:

Article Title: Effectorless Fc-fusion improves FLT3L drug-like properties for cancer immunotherapy combinations
Article Snippet: CD34+ human cord blood stem cells (StemCell Technologies) were seeded at 5000 cells per well in 96-well U-bottom plates (Costar) in 100 μL expansion media (StemSpan media–StemCell Technologies, 10% FBS, 40 ng/mL IL-3, 200 ng/mL SCF, 100 ng/mL TPO–cytokines from Peprotech).

Article Title: Perturbing LSD1 and WNT rewires transcription to synergistically induce AML differentiation
Article Snippet: Human cord blood CD34 + cells (200-0000, StemCell Technologies) were plated in methylcellulose (MethoCult H4534 Classic, StemCell Technologies).

Article Title: Utility of CK8/18 in identifying circulating tumor cells derived from lesions in patients with non-small cell lung cancer
Article Snippet: Human stem cells (Human Cord Blood CD34 + cell) were obtained from Stem Cell Technologies (Vancouver, Canada).

Article Title: Quantification of intrinsic regulatory factors refines human hematopoietic progenitor definitions and reveals early erythroid lineage priming.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies All antibodies used for cytometry Various Table S1 Biological samples Human BM CD34+ cells bluebird bio n/a Human mPB CD34+ cells bluebird bio n/a Human MNC from FL AllCells n/a Cord Blood CD34+ cells STEMCELL Technologies n/a Chemicals, peptides, and recombinant proteins Ficoll-Paque Plus GE Healthcare Cat#300-25 Bovine Serum Album (BSA) Heat-shock Treated Thermo Fisher Scientific Cat#BP1600-100 Human TruStain FcX (Fc Receptor Blocking Solution) BioLegend Cat#422302 Cell-ID Intercalator-Ir Standard BioTools Inc. Cat#201192A Cell-ID Cisplatin Standard BioTools Inc. Cat#201064 Saponin from quillaja bark Sigma-Aldrich Cat#S7900-25G Calibration Beads, EQ, Four Element Standard BioTools Inc. Cat#201078 7-AAD Viability Staining Solution BioLegend Cat#420404 Benzonase nuclease Sigma-Aldrich Cat#E1014-25KU Deposited data Mass cytometry and integration data and code This study https://doi.org/10.5281/zenodo.13968159 ATAC-seq processed data and code This study https://doi.org/10.5281/zenodo.8266662 ATAC-seq sequence data This study GEO: GSE241229 Experimental models: Organisms/strains NSG mice (NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ) Jackson Laboratory N/A Recombinant DNA EGFP-VSVg-ProtC-AU1 Addgene N/A mCherry-StrepTagII-FLAG-AU1 Addgene N/A Software and algorithms RStudio RStudio rstudio.com Cytobank BeckmanCoulter beckman.com CellEngine Primity Bio cellengine.com MATLAB MathWorks mathworks.co Cell Reports 44, 115913, July 22, 2025 19

Article Title: Effectorless Fc-fusion improves FLT3L drug-like properties for cancer immunotherapy combinations.
Article Snippet: 41 CD34+ human cord blood stem cells (StemCell Technologies) were seeded at 5000 cells per well in 96-well U-bottom plates (Costar) in 100 μL expansion media (StemSpan media–StemCell Technologies, 10% FBS, 40 ng/mL IL-3, 200 ng/mL SCF, 100 ng/mL TPO–cytokines from Peprotech).

Article Title: Lactate dehydrogenase A-coupled NAD + regeneration is critical for acute myeloid leukemia cell survival
Article Snippet: CD34 + human cord blood-derived cells were obtained from STEMCELL Technologies and were maintained in StemSpan SFEM-II with CD34 supplements (STEMCELL Technologies).

Article Title: Perturbing LSD1 and WNT rewires transcription to synergistically induce AML differentiation.
Article Snippet: Human cord blood CD34+ cells (200-0000, StemCell Technologies) were plated in methylcellulose (MethoCult H4534 Classic, StemCell Technologies).

Article Title: Lactate dehydrogenase A-coupled NAD + regeneration is critical for acute myeloid leukemia cell survival.
Article Snippet: CD34+ human cord blood-derived cells were obtained from STEMCELL Technologies and were maintained in StemSpan SFEM-II with CD34 supplements (STEMCELL Technologies).

Sequencing:

Article Title: Effectorless Fc-fusion improves FLT3L drug-like properties for cancer immunotherapy combinations
Article Snippet: CD34+ human cord blood stem cells (StemCell Technologies) were seeded at 5000 cells per well in 96-well U-bottom plates (Costar) in 100 μL expansion media (StemSpan media–StemCell Technologies, 10% FBS, 40 ng/mL IL-3, 200 ng/mL SCF, 100 ng/mL TPO–cytokines from Peprotech).

Article Title: Perturbing LSD1 and WNT rewires transcription to synergistically induce AML differentiation
Article Snippet: Human cord blood CD34 + cells (200-0000, StemCell Technologies) were plated in methylcellulose (MethoCult H4534 Classic, StemCell Technologies).

Article Title: Utility of CK8/18 in identifying circulating tumor cells derived from lesions in patients with non-small cell lung cancer
Article Snippet: Human stem cells (Human Cord Blood CD34 + cell) were obtained from Stem Cell Technologies (Vancouver, Canada).

Article Title: Quantification of intrinsic regulatory factors refines human hematopoietic progenitor definitions and reveals early erythroid lineage priming.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies All antibodies used for cytometry Various Table S1 Biological samples Human BM CD34+ cells bluebird bio n/a Human mPB CD34+ cells bluebird bio n/a Human MNC from FL AllCells n/a Cord Blood CD34+ cells STEMCELL Technologies n/a Chemicals, peptides, and recombinant proteins Ficoll-Paque Plus GE Healthcare Cat#300-25 Bovine Serum Album (BSA) Heat-shock Treated Thermo Fisher Scientific Cat#BP1600-100 Human TruStain FcX (Fc Receptor Blocking Solution) BioLegend Cat#422302 Cell-ID Intercalator-Ir Standard BioTools Inc. Cat#201192A Cell-ID Cisplatin Standard BioTools Inc. Cat#201064 Saponin from quillaja bark Sigma-Aldrich Cat#S7900-25G Calibration Beads, EQ, Four Element Standard BioTools Inc. Cat#201078 7-AAD Viability Staining Solution BioLegend Cat#420404 Benzonase nuclease Sigma-Aldrich Cat#E1014-25KU Deposited data Mass cytometry and integration data and code This study https://doi.org/10.5281/zenodo.13968159 ATAC-seq processed data and code This study https://doi.org/10.5281/zenodo.8266662 ATAC-seq sequence data This study GEO: GSE241229 Experimental models: Organisms/strains NSG mice (NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ) Jackson Laboratory N/A Recombinant DNA EGFP-VSVg-ProtC-AU1 Addgene N/A mCherry-StrepTagII-FLAG-AU1 Addgene N/A Software and algorithms RStudio RStudio rstudio.com Cytobank BeckmanCoulter beckman.com CellEngine Primity Bio cellengine.com MATLAB MathWorks mathworks.co Cell Reports 44, 115913, July 22, 2025 19

Article Title: Effectorless Fc-fusion improves FLT3L drug-like properties for cancer immunotherapy combinations.
Article Snippet: 41 CD34+ human cord blood stem cells (StemCell Technologies) were seeded at 5000 cells per well in 96-well U-bottom plates (Costar) in 100 μL expansion media (StemSpan media–StemCell Technologies, 10% FBS, 40 ng/mL IL-3, 200 ng/mL SCF, 100 ng/mL TPO–cytokines from Peprotech).

Article Title: Lactate dehydrogenase A-coupled NAD + regeneration is critical for acute myeloid leukemia cell survival
Article Snippet: CD34 + human cord blood-derived cells were obtained from STEMCELL Technologies and were maintained in StemSpan SFEM-II with CD34 supplements (STEMCELL Technologies).

Article Title: Perturbing LSD1 and WNT rewires transcription to synergistically induce AML differentiation.
Article Snippet: Human cord blood CD34+ cells (200-0000, StemCell Technologies) were plated in methylcellulose (MethoCult H4534 Classic, StemCell Technologies).

Article Title: Lactate dehydrogenase A-coupled NAD + regeneration is critical for acute myeloid leukemia cell survival.
Article Snippet: CD34+ human cord blood-derived cells were obtained from STEMCELL Technologies and were maintained in StemSpan SFEM-II with CD34 supplements (STEMCELL Technologies).

Software:

Article Title: Effectorless Fc-fusion improves FLT3L drug-like properties for cancer immunotherapy combinations
Article Snippet: CD34+ human cord blood stem cells (StemCell Technologies) were seeded at 5000 cells per well in 96-well U-bottom plates (Costar) in 100 μL expansion media (StemSpan media–StemCell Technologies, 10% FBS, 40 ng/mL IL-3, 200 ng/mL SCF, 100 ng/mL TPO–cytokines from Peprotech).

Article Title: Perturbing LSD1 and WNT rewires transcription to synergistically induce AML differentiation
Article Snippet: Human cord blood CD34 + cells (200-0000, StemCell Technologies) were plated in methylcellulose (MethoCult H4534 Classic, StemCell Technologies).

Article Title: Utility of CK8/18 in identifying circulating tumor cells derived from lesions in patients with non-small cell lung cancer
Article Snippet: Human stem cells (Human Cord Blood CD34 + cell) were obtained from Stem Cell Technologies (Vancouver, Canada).

Article Title: Quantification of intrinsic regulatory factors refines human hematopoietic progenitor definitions and reveals early erythroid lineage priming.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies All antibodies used for cytometry Various Table S1 Biological samples Human BM CD34+ cells bluebird bio n/a Human mPB CD34+ cells bluebird bio n/a Human MNC from FL AllCells n/a Cord Blood CD34+ cells STEMCELL Technologies n/a Chemicals, peptides, and recombinant proteins Ficoll-Paque Plus GE Healthcare Cat#300-25 Bovine Serum Album (BSA) Heat-shock Treated Thermo Fisher Scientific Cat#BP1600-100 Human TruStain FcX (Fc Receptor Blocking Solution) BioLegend Cat#422302 Cell-ID Intercalator-Ir Standard BioTools Inc. Cat#201192A Cell-ID Cisplatin Standard BioTools Inc. Cat#201064 Saponin from quillaja bark Sigma-Aldrich Cat#S7900-25G Calibration Beads, EQ, Four Element Standard BioTools Inc. Cat#201078 7-AAD Viability Staining Solution BioLegend Cat#420404 Benzonase nuclease Sigma-Aldrich Cat#E1014-25KU Deposited data Mass cytometry and integration data and code This study https://doi.org/10.5281/zenodo.13968159 ATAC-seq processed data and code This study https://doi.org/10.5281/zenodo.8266662 ATAC-seq sequence data This study GEO: GSE241229 Experimental models: Organisms/strains NSG mice (NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ) Jackson Laboratory N/A Recombinant DNA EGFP-VSVg-ProtC-AU1 Addgene N/A mCherry-StrepTagII-FLAG-AU1 Addgene N/A Software and algorithms RStudio RStudio rstudio.com Cytobank BeckmanCoulter beckman.com CellEngine Primity Bio cellengine.com MATLAB MathWorks mathworks.co Cell Reports 44, 115913, July 22, 2025 19

Article Title: Effectorless Fc-fusion improves FLT3L drug-like properties for cancer immunotherapy combinations.
Article Snippet: 41 CD34+ human cord blood stem cells (StemCell Technologies) were seeded at 5000 cells per well in 96-well U-bottom plates (Costar) in 100 μL expansion media (StemSpan media–StemCell Technologies, 10% FBS, 40 ng/mL IL-3, 200 ng/mL SCF, 100 ng/mL TPO–cytokines from Peprotech).

Article Title: Lactate dehydrogenase A-coupled NAD + regeneration is critical for acute myeloid leukemia cell survival
Article Snippet: CD34 + human cord blood-derived cells were obtained from STEMCELL Technologies and were maintained in StemSpan SFEM-II with CD34 supplements (STEMCELL Technologies).

Article Title: Perturbing LSD1 and WNT rewires transcription to synergistically induce AML differentiation.
Article Snippet: Human cord blood CD34+ cells (200-0000, StemCell Technologies) were plated in methylcellulose (MethoCult H4534 Classic, StemCell Technologies).

Article Title: Lactate dehydrogenase A-coupled NAD + regeneration is critical for acute myeloid leukemia cell survival.
Article Snippet: CD34+ human cord blood-derived cells were obtained from STEMCELL Technologies and were maintained in StemSpan SFEM-II with CD34 supplements (STEMCELL Technologies).

RNA Sequencing:

Article Title: Effectorless Fc-fusion improves FLT3L drug-like properties for cancer immunotherapy combinations
Article Snippet: CD34+ human cord blood stem cells (StemCell Technologies) were seeded at 5000 cells per well in 96-well U-bottom plates (Costar) in 100 μL expansion media (StemSpan media–StemCell Technologies, 10% FBS, 40 ng/mL IL-3, 200 ng/mL SCF, 100 ng/mL TPO–cytokines from Peprotech).

Article Title: Perturbing LSD1 and WNT rewires transcription to synergistically induce AML differentiation
Article Snippet: Human cord blood CD34 + cells (200-0000, StemCell Technologies) were plated in methylcellulose (MethoCult H4534 Classic, StemCell Technologies).

Article Title: Utility of CK8/18 in identifying circulating tumor cells derived from lesions in patients with non-small cell lung cancer
Article Snippet: Human stem cells (Human Cord Blood CD34 + cell) were obtained from Stem Cell Technologies (Vancouver, Canada).

Article Title: Quantification of intrinsic regulatory factors refines human hematopoietic progenitor definitions and reveals early erythroid lineage priming.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies All antibodies used for cytometry Various Table S1 Biological samples Human BM CD34+ cells bluebird bio n/a Human mPB CD34+ cells bluebird bio n/a Human MNC from FL AllCells n/a Cord Blood CD34+ cells STEMCELL Technologies n/a Chemicals, peptides, and recombinant proteins Ficoll-Paque Plus GE Healthcare Cat#300-25 Bovine Serum Album (BSA) Heat-shock Treated Thermo Fisher Scientific Cat#BP1600-100 Human TruStain FcX (Fc Receptor Blocking Solution) BioLegend Cat#422302 Cell-ID Intercalator-Ir Standard BioTools Inc. Cat#201192A Cell-ID Cisplatin Standard BioTools Inc. Cat#201064 Saponin from quillaja bark Sigma-Aldrich Cat#S7900-25G Calibration Beads, EQ, Four Element Standard BioTools Inc. Cat#201078 7-AAD Viability Staining Solution BioLegend Cat#420404 Benzonase nuclease Sigma-Aldrich Cat#E1014-25KU Deposited data Mass cytometry and integration data and code This study https://doi.org/10.5281/zenodo.13968159 ATAC-seq processed data and code This study https://doi.org/10.5281/zenodo.8266662 ATAC-seq sequence data This study GEO: GSE241229 Experimental models: Organisms/strains NSG mice (NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ) Jackson Laboratory N/A Recombinant DNA EGFP-VSVg-ProtC-AU1 Addgene N/A mCherry-StrepTagII-FLAG-AU1 Addgene N/A Software and algorithms RStudio RStudio rstudio.com Cytobank BeckmanCoulter beckman.com CellEngine Primity Bio cellengine.com MATLAB MathWorks mathworks.co Cell Reports 44, 115913, July 22, 2025 19

Article Title: Effectorless Fc-fusion improves FLT3L drug-like properties for cancer immunotherapy combinations.
Article Snippet: 41 CD34+ human cord blood stem cells (StemCell Technologies) were seeded at 5000 cells per well in 96-well U-bottom plates (Costar) in 100 μL expansion media (StemSpan media–StemCell Technologies, 10% FBS, 40 ng/mL IL-3, 200 ng/mL SCF, 100 ng/mL TPO–cytokines from Peprotech).

Article Title: Lactate dehydrogenase A-coupled NAD + regeneration is critical for acute myeloid leukemia cell survival
Article Snippet: CD34 + human cord blood-derived cells were obtained from STEMCELL Technologies and were maintained in StemSpan SFEM-II with CD34 supplements (STEMCELL Technologies).

Article Title: Perturbing LSD1 and WNT rewires transcription to synergistically induce AML differentiation.
Article Snippet: Human cord blood CD34+ cells (200-0000, StemCell Technologies) were plated in methylcellulose (MethoCult H4534 Classic, StemCell Technologies).

Article Title: Lactate dehydrogenase A-coupled NAD + regeneration is critical for acute myeloid leukemia cell survival.
Article Snippet: CD34+ human cord blood-derived cells were obtained from STEMCELL Technologies and were maintained in StemSpan SFEM-II with CD34 supplements (STEMCELL Technologies).

Western Blot:

Article Title: Effectorless Fc-fusion improves FLT3L drug-like properties for cancer immunotherapy combinations
Article Snippet: CD34+ human cord blood stem cells (StemCell Technologies) were seeded at 5000 cells per well in 96-well U-bottom plates (Costar) in 100 μL expansion media (StemSpan media–StemCell Technologies, 10% FBS, 40 ng/mL IL-3, 200 ng/mL SCF, 100 ng/mL TPO–cytokines from Peprotech).

Article Title: Perturbing LSD1 and WNT rewires transcription to synergistically induce AML differentiation
Article Snippet: Human cord blood CD34 + cells (200-0000, StemCell Technologies) were plated in methylcellulose (MethoCult H4534 Classic, StemCell Technologies).

Article Title: Utility of CK8/18 in identifying circulating tumor cells derived from lesions in patients with non-small cell lung cancer
Article Snippet: Human stem cells (Human Cord Blood CD34 + cell) were obtained from Stem Cell Technologies (Vancouver, Canada).

Article Title: Quantification of intrinsic regulatory factors refines human hematopoietic progenitor definitions and reveals early erythroid lineage priming.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies All antibodies used for cytometry Various Table S1 Biological samples Human BM CD34+ cells bluebird bio n/a Human mPB CD34+ cells bluebird bio n/a Human MNC from FL AllCells n/a Cord Blood CD34+ cells STEMCELL Technologies n/a Chemicals, peptides, and recombinant proteins Ficoll-Paque Plus GE Healthcare Cat#300-25 Bovine Serum Album (BSA) Heat-shock Treated Thermo Fisher Scientific Cat#BP1600-100 Human TruStain FcX (Fc Receptor Blocking Solution) BioLegend Cat#422302 Cell-ID Intercalator-Ir Standard BioTools Inc. Cat#201192A Cell-ID Cisplatin Standard BioTools Inc. Cat#201064 Saponin from quillaja bark Sigma-Aldrich Cat#S7900-25G Calibration Beads, EQ, Four Element Standard BioTools Inc. Cat#201078 7-AAD Viability Staining Solution BioLegend Cat#420404 Benzonase nuclease Sigma-Aldrich Cat#E1014-25KU Deposited data Mass cytometry and integration data and code This study https://doi.org/10.5281/zenodo.13968159 ATAC-seq processed data and code This study https://doi.org/10.5281/zenodo.8266662 ATAC-seq sequence data This study GEO: GSE241229 Experimental models: Organisms/strains NSG mice (NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ) Jackson Laboratory N/A Recombinant DNA EGFP-VSVg-ProtC-AU1 Addgene N/A mCherry-StrepTagII-FLAG-AU1 Addgene N/A Software and algorithms RStudio RStudio rstudio.com Cytobank BeckmanCoulter beckman.com CellEngine Primity Bio cellengine.com MATLAB MathWorks mathworks.co Cell Reports 44, 115913, July 22, 2025 19

Article Title: Effectorless Fc-fusion improves FLT3L drug-like properties for cancer immunotherapy combinations.
Article Snippet: 41 CD34+ human cord blood stem cells (StemCell Technologies) were seeded at 5000 cells per well in 96-well U-bottom plates (Costar) in 100 μL expansion media (StemSpan media–StemCell Technologies, 10% FBS, 40 ng/mL IL-3, 200 ng/mL SCF, 100 ng/mL TPO–cytokines from Peprotech).

Article Title: Lactate dehydrogenase A-coupled NAD + regeneration is critical for acute myeloid leukemia cell survival
Article Snippet: CD34 + human cord blood-derived cells were obtained from STEMCELL Technologies and were maintained in StemSpan SFEM-II with CD34 supplements (STEMCELL Technologies).

Article Title: Perturbing LSD1 and WNT rewires transcription to synergistically induce AML differentiation.
Article Snippet: Human cord blood CD34+ cells (200-0000, StemCell Technologies) were plated in methylcellulose (MethoCult H4534 Classic, StemCell Technologies).

Article Title: Lactate dehydrogenase A-coupled NAD + regeneration is critical for acute myeloid leukemia cell survival.
Article Snippet: CD34+ human cord blood-derived cells were obtained from STEMCELL Technologies and were maintained in StemSpan SFEM-II with CD34 supplements (STEMCELL Technologies).

Expressing:

Article Title: Effectorless Fc-fusion improves FLT3L drug-like properties for cancer immunotherapy combinations
Article Snippet: CD34+ human cord blood stem cells (StemCell Technologies) were seeded at 5000 cells per well in 96-well U-bottom plates (Costar) in 100 μL expansion media (StemSpan media–StemCell Technologies, 10% FBS, 40 ng/mL IL-3, 200 ng/mL SCF, 100 ng/mL TPO–cytokines from Peprotech).

Article Title: Perturbing LSD1 and WNT rewires transcription to synergistically induce AML differentiation
Article Snippet: Human cord blood CD34 + cells (200-0000, StemCell Technologies) were plated in methylcellulose (MethoCult H4534 Classic, StemCell Technologies).

Article Title: Utility of CK8/18 in identifying circulating tumor cells derived from lesions in patients with non-small cell lung cancer
Article Snippet: Human stem cells (Human Cord Blood CD34 + cell) were obtained from Stem Cell Technologies (Vancouver, Canada).

Article Title: Quantification of intrinsic regulatory factors refines human hematopoietic progenitor definitions and reveals early erythroid lineage priming.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies All antibodies used for cytometry Various Table S1 Biological samples Human BM CD34+ cells bluebird bio n/a Human mPB CD34+ cells bluebird bio n/a Human MNC from FL AllCells n/a Cord Blood CD34+ cells STEMCELL Technologies n/a Chemicals, peptides, and recombinant proteins Ficoll-Paque Plus GE Healthcare Cat#300-25 Bovine Serum Album (BSA) Heat-shock Treated Thermo Fisher Scientific Cat#BP1600-100 Human TruStain FcX (Fc Receptor Blocking Solution) BioLegend Cat#422302 Cell-ID Intercalator-Ir Standard BioTools Inc. Cat#201192A Cell-ID Cisplatin Standard BioTools Inc. Cat#201064 Saponin from quillaja bark Sigma-Aldrich Cat#S7900-25G Calibration Beads, EQ, Four Element Standard BioTools Inc. Cat#201078 7-AAD Viability Staining Solution BioLegend Cat#420404 Benzonase nuclease Sigma-Aldrich Cat#E1014-25KU Deposited data Mass cytometry and integration data and code This study https://doi.org/10.5281/zenodo.13968159 ATAC-seq processed data and code This study https://doi.org/10.5281/zenodo.8266662 ATAC-seq sequence data This study GEO: GSE241229 Experimental models: Organisms/strains NSG mice (NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ) Jackson Laboratory N/A Recombinant DNA EGFP-VSVg-ProtC-AU1 Addgene N/A mCherry-StrepTagII-FLAG-AU1 Addgene N/A Software and algorithms RStudio RStudio rstudio.com Cytobank BeckmanCoulter beckman.com CellEngine Primity Bio cellengine.com MATLAB MathWorks mathworks.co Cell Reports 44, 115913, July 22, 2025 19

Article Title: Effectorless Fc-fusion improves FLT3L drug-like properties for cancer immunotherapy combinations.
Article Snippet: 41 CD34+ human cord blood stem cells (StemCell Technologies) were seeded at 5000 cells per well in 96-well U-bottom plates (Costar) in 100 μL expansion media (StemSpan media–StemCell Technologies, 10% FBS, 40 ng/mL IL-3, 200 ng/mL SCF, 100 ng/mL TPO–cytokines from Peprotech).

Article Title: Lactate dehydrogenase A-coupled NAD + regeneration is critical for acute myeloid leukemia cell survival
Article Snippet: CD34 + human cord blood-derived cells were obtained from STEMCELL Technologies and were maintained in StemSpan SFEM-II with CD34 supplements (STEMCELL Technologies).

Article Title: Perturbing LSD1 and WNT rewires transcription to synergistically induce AML differentiation.
Article Snippet: Human cord blood CD34+ cells (200-0000, StemCell Technologies) were plated in methylcellulose (MethoCult H4534 Classic, StemCell Technologies).

Article Title: Lactate dehydrogenase A-coupled NAD + regeneration is critical for acute myeloid leukemia cell survival.
Article Snippet: CD34+ human cord blood-derived cells were obtained from STEMCELL Technologies and were maintained in StemSpan SFEM-II with CD34 supplements (STEMCELL Technologies).

Two Tailed Test:

Article Title: Effectorless Fc-fusion improves FLT3L drug-like properties for cancer immunotherapy combinations
Article Snippet: CD34+ human cord blood stem cells (StemCell Technologies) were seeded at 5000 cells per well in 96-well U-bottom plates (Costar) in 100 μL expansion media (StemSpan media–StemCell Technologies, 10% FBS, 40 ng/mL IL-3, 200 ng/mL SCF, 100 ng/mL TPO–cytokines from Peprotech).

Article Title: Perturbing LSD1 and WNT rewires transcription to synergistically induce AML differentiation
Article Snippet: Human cord blood CD34 + cells (200-0000, StemCell Technologies) were plated in methylcellulose (MethoCult H4534 Classic, StemCell Technologies).

Article Title: Utility of CK8/18 in identifying circulating tumor cells derived from lesions in patients with non-small cell lung cancer
Article Snippet: Human stem cells (Human Cord Blood CD34 + cell) were obtained from Stem Cell Technologies (Vancouver, Canada).

Article Title: Quantification of intrinsic regulatory factors refines human hematopoietic progenitor definitions and reveals early erythroid lineage priming.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies All antibodies used for cytometry Various Table S1 Biological samples Human BM CD34+ cells bluebird bio n/a Human mPB CD34+ cells bluebird bio n/a Human MNC from FL AllCells n/a Cord Blood CD34+ cells STEMCELL Technologies n/a Chemicals, peptides, and recombinant proteins Ficoll-Paque Plus GE Healthcare Cat#300-25 Bovine Serum Album (BSA) Heat-shock Treated Thermo Fisher Scientific Cat#BP1600-100 Human TruStain FcX (Fc Receptor Blocking Solution) BioLegend Cat#422302 Cell-ID Intercalator-Ir Standard BioTools Inc. Cat#201192A Cell-ID Cisplatin Standard BioTools Inc. Cat#201064 Saponin from quillaja bark Sigma-Aldrich Cat#S7900-25G Calibration Beads, EQ, Four Element Standard BioTools Inc. Cat#201078 7-AAD Viability Staining Solution BioLegend Cat#420404 Benzonase nuclease Sigma-Aldrich Cat#E1014-25KU Deposited data Mass cytometry and integration data and code This study https://doi.org/10.5281/zenodo.13968159 ATAC-seq processed data and code This study https://doi.org/10.5281/zenodo.8266662 ATAC-seq sequence data This study GEO: GSE241229 Experimental models: Organisms/strains NSG mice (NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ) Jackson Laboratory N/A Recombinant DNA EGFP-VSVg-ProtC-AU1 Addgene N/A mCherry-StrepTagII-FLAG-AU1 Addgene N/A Software and algorithms RStudio RStudio rstudio.com Cytobank BeckmanCoulter beckman.com CellEngine Primity Bio cellengine.com MATLAB MathWorks mathworks.co Cell Reports 44, 115913, July 22, 2025 19

Article Title: Effectorless Fc-fusion improves FLT3L drug-like properties for cancer immunotherapy combinations.
Article Snippet: 41 CD34+ human cord blood stem cells (StemCell Technologies) were seeded at 5000 cells per well in 96-well U-bottom plates (Costar) in 100 μL expansion media (StemSpan media–StemCell Technologies, 10% FBS, 40 ng/mL IL-3, 200 ng/mL SCF, 100 ng/mL TPO–cytokines from Peprotech).

Article Title: Lactate dehydrogenase A-coupled NAD + regeneration is critical for acute myeloid leukemia cell survival
Article Snippet: CD34 + human cord blood-derived cells were obtained from STEMCELL Technologies and were maintained in StemSpan SFEM-II with CD34 supplements (STEMCELL Technologies).

Article Title: Perturbing LSD1 and WNT rewires transcription to synergistically induce AML differentiation.
Article Snippet: Human cord blood CD34+ cells (200-0000, StemCell Technologies) were plated in methylcellulose (MethoCult H4534 Classic, StemCell Technologies).

Article Title: Lactate dehydrogenase A-coupled NAD + regeneration is critical for acute myeloid leukemia cell survival.
Article Snippet: CD34+ human cord blood-derived cells were obtained from STEMCELL Technologies and were maintained in StemSpan SFEM-II with CD34 supplements (STEMCELL Technologies).

Dot Blot:

Article Title: Effectorless Fc-fusion improves FLT3L drug-like properties for cancer immunotherapy combinations
Article Snippet: CD34+ human cord blood stem cells (StemCell Technologies) were seeded at 5000 cells per well in 96-well U-bottom plates (Costar) in 100 μL expansion media (StemSpan media–StemCell Technologies, 10% FBS, 40 ng/mL IL-3, 200 ng/mL SCF, 100 ng/mL TPO–cytokines from Peprotech).

Article Title: Perturbing LSD1 and WNT rewires transcription to synergistically induce AML differentiation
Article Snippet: Human cord blood CD34 + cells (200-0000, StemCell Technologies) were plated in methylcellulose (MethoCult H4534 Classic, StemCell Technologies).

Article Title: Utility of CK8/18 in identifying circulating tumor cells derived from lesions in patients with non-small cell lung cancer
Article Snippet: Human stem cells (Human Cord Blood CD34 + cell) were obtained from Stem Cell Technologies (Vancouver, Canada).

Article Title: Quantification of intrinsic regulatory factors refines human hematopoietic progenitor definitions and reveals early erythroid lineage priming.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies All antibodies used for cytometry Various Table S1 Biological samples Human BM CD34+ cells bluebird bio n/a Human mPB CD34+ cells bluebird bio n/a Human MNC from FL AllCells n/a Cord Blood CD34+ cells STEMCELL Technologies n/a Chemicals, peptides, and recombinant proteins Ficoll-Paque Plus GE Healthcare Cat#300-25 Bovine Serum Album (BSA) Heat-shock Treated Thermo Fisher Scientific Cat#BP1600-100 Human TruStain FcX (Fc Receptor Blocking Solution) BioLegend Cat#422302 Cell-ID Intercalator-Ir Standard BioTools Inc. Cat#201192A Cell-ID Cisplatin Standard BioTools Inc. Cat#201064 Saponin from quillaja bark Sigma-Aldrich Cat#S7900-25G Calibration Beads, EQ, Four Element Standard BioTools Inc. Cat#201078 7-AAD Viability Staining Solution BioLegend Cat#420404 Benzonase nuclease Sigma-Aldrich Cat#E1014-25KU Deposited data Mass cytometry and integration data and code This study https://doi.org/10.5281/zenodo.13968159 ATAC-seq processed data and code This study https://doi.org/10.5281/zenodo.8266662 ATAC-seq sequence data This study GEO: GSE241229 Experimental models: Organisms/strains NSG mice (NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ) Jackson Laboratory N/A Recombinant DNA EGFP-VSVg-ProtC-AU1 Addgene N/A mCherry-StrepTagII-FLAG-AU1 Addgene N/A Software and algorithms RStudio RStudio rstudio.com Cytobank BeckmanCoulter beckman.com CellEngine Primity Bio cellengine.com MATLAB MathWorks mathworks.co Cell Reports 44, 115913, July 22, 2025 19

Article Title: Effectorless Fc-fusion improves FLT3L drug-like properties for cancer immunotherapy combinations.
Article Snippet: 41 CD34+ human cord blood stem cells (StemCell Technologies) were seeded at 5000 cells per well in 96-well U-bottom plates (Costar) in 100 μL expansion media (StemSpan media–StemCell Technologies, 10% FBS, 40 ng/mL IL-3, 200 ng/mL SCF, 100 ng/mL TPO–cytokines from Peprotech).

Article Title: Lactate dehydrogenase A-coupled NAD + regeneration is critical for acute myeloid leukemia cell survival
Article Snippet: CD34 + human cord blood-derived cells were obtained from STEMCELL Technologies and were maintained in StemSpan SFEM-II with CD34 supplements (STEMCELL Technologies).

Article Title: Perturbing LSD1 and WNT rewires transcription to synergistically induce AML differentiation.
Article Snippet: Human cord blood CD34+ cells (200-0000, StemCell Technologies) were plated in methylcellulose (MethoCult H4534 Classic, StemCell Technologies).

Article Title: Lactate dehydrogenase A-coupled NAD + regeneration is critical for acute myeloid leukemia cell survival.
Article Snippet: CD34+ human cord blood-derived cells were obtained from STEMCELL Technologies and were maintained in StemSpan SFEM-II with CD34 supplements (STEMCELL Technologies).

Cell Culture:

Article Title: Effectorless Fc-fusion improves FLT3L drug-like properties for cancer immunotherapy combinations
Article Snippet: CD34+ human cord blood stem cells (StemCell Technologies) were seeded at 5000 cells per well in 96-well U-bottom plates (Costar) in 100 μL expansion media (StemSpan media–StemCell Technologies, 10% FBS, 40 ng/mL IL-3, 200 ng/mL SCF, 100 ng/mL TPO–cytokines from Peprotech).

Article Title: Perturbing LSD1 and WNT rewires transcription to synergistically induce AML differentiation
Article Snippet: Human cord blood CD34 + cells (200-0000, StemCell Technologies) were plated in methylcellulose (MethoCult H4534 Classic, StemCell Technologies).

Article Title: Utility of CK8/18 in identifying circulating tumor cells derived from lesions in patients with non-small cell lung cancer
Article Snippet: Human stem cells (Human Cord Blood CD34 + cell) were obtained from Stem Cell Technologies (Vancouver, Canada).

Article Title: Quantification of intrinsic regulatory factors refines human hematopoietic progenitor definitions and reveals early erythroid lineage priming.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies All antibodies used for cytometry Various Table S1 Biological samples Human BM CD34+ cells bluebird bio n/a Human mPB CD34+ cells bluebird bio n/a Human MNC from FL AllCells n/a Cord Blood CD34+ cells STEMCELL Technologies n/a Chemicals, peptides, and recombinant proteins Ficoll-Paque Plus GE Healthcare Cat#300-25 Bovine Serum Album (BSA) Heat-shock Treated Thermo Fisher Scientific Cat#BP1600-100 Human TruStain FcX (Fc Receptor Blocking Solution) BioLegend Cat#422302 Cell-ID Intercalator-Ir Standard BioTools Inc. Cat#201192A Cell-ID Cisplatin Standard BioTools Inc. Cat#201064 Saponin from quillaja bark Sigma-Aldrich Cat#S7900-25G Calibration Beads, EQ, Four Element Standard BioTools Inc. Cat#201078 7-AAD Viability Staining Solution BioLegend Cat#420404 Benzonase nuclease Sigma-Aldrich Cat#E1014-25KU Deposited data Mass cytometry and integration data and code This study https://doi.org/10.5281/zenodo.13968159 ATAC-seq processed data and code This study https://doi.org/10.5281/zenodo.8266662 ATAC-seq sequence data This study GEO: GSE241229 Experimental models: Organisms/strains NSG mice (NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ) Jackson Laboratory N/A Recombinant DNA EGFP-VSVg-ProtC-AU1 Addgene N/A mCherry-StrepTagII-FLAG-AU1 Addgene N/A Software and algorithms RStudio RStudio rstudio.com Cytobank BeckmanCoulter beckman.com CellEngine Primity Bio cellengine.com MATLAB MathWorks mathworks.co Cell Reports 44, 115913, July 22, 2025 19

Article Title: Effectorless Fc-fusion improves FLT3L drug-like properties for cancer immunotherapy combinations.
Article Snippet: 41 CD34+ human cord blood stem cells (StemCell Technologies) were seeded at 5000 cells per well in 96-well U-bottom plates (Costar) in 100 μL expansion media (StemSpan media–StemCell Technologies, 10% FBS, 40 ng/mL IL-3, 200 ng/mL SCF, 100 ng/mL TPO–cytokines from Peprotech).

Article Title: Lactate dehydrogenase A-coupled NAD + regeneration is critical for acute myeloid leukemia cell survival
Article Snippet: CD34 + human cord blood-derived cells were obtained from STEMCELL Technologies and were maintained in StemSpan SFEM-II with CD34 supplements (STEMCELL Technologies).

Article Title: Perturbing LSD1 and WNT rewires transcription to synergistically induce AML differentiation.
Article Snippet: Human cord blood CD34+ cells (200-0000, StemCell Technologies) were plated in methylcellulose (MethoCult H4534 Classic, StemCell Technologies).

Article Title: Lactate dehydrogenase A-coupled NAD + regeneration is critical for acute myeloid leukemia cell survival.
Article Snippet: CD34+ human cord blood-derived cells were obtained from STEMCELL Technologies and were maintained in StemSpan SFEM-II with CD34 supplements (STEMCELL Technologies).

Mutagenesis:

Article Title: Effectorless Fc-fusion improves FLT3L drug-like properties for cancer immunotherapy combinations
Article Snippet: CD34+ human cord blood stem cells (StemCell Technologies) were seeded at 5000 cells per well in 96-well U-bottom plates (Costar) in 100 μL expansion media (StemSpan media–StemCell Technologies, 10% FBS, 40 ng/mL IL-3, 200 ng/mL SCF, 100 ng/mL TPO–cytokines from Peprotech).

Article Title: Perturbing LSD1 and WNT rewires transcription to synergistically induce AML differentiation
Article Snippet: Human cord blood CD34 + cells (200-0000, StemCell Technologies) were plated in methylcellulose (MethoCult H4534 Classic, StemCell Technologies).

Article Title: Utility of CK8/18 in identifying circulating tumor cells derived from lesions in patients with non-small cell lung cancer
Article Snippet: Human stem cells (Human Cord Blood CD34 + cell) were obtained from Stem Cell Technologies (Vancouver, Canada).

Article Title: Quantification of intrinsic regulatory factors refines human hematopoietic progenitor definitions and reveals early erythroid lineage priming.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies All antibodies used for cytometry Various Table S1 Biological samples Human BM CD34+ cells bluebird bio n/a Human mPB CD34+ cells bluebird bio n/a Human MNC from FL AllCells n/a Cord Blood CD34+ cells STEMCELL Technologies n/a Chemicals, peptides, and recombinant proteins Ficoll-Paque Plus GE Healthcare Cat#300-25 Bovine Serum Album (BSA) Heat-shock Treated Thermo Fisher Scientific Cat#BP1600-100 Human TruStain FcX (Fc Receptor Blocking Solution) BioLegend Cat#422302 Cell-ID Intercalator-Ir Standard BioTools Inc. Cat#201192A Cell-ID Cisplatin Standard BioTools Inc. Cat#201064 Saponin from quillaja bark Sigma-Aldrich Cat#S7900-25G Calibration Beads, EQ, Four Element Standard BioTools Inc. Cat#201078 7-AAD Viability Staining Solution BioLegend Cat#420404 Benzonase nuclease Sigma-Aldrich Cat#E1014-25KU Deposited data Mass cytometry and integration data and code This study https://doi.org/10.5281/zenodo.13968159 ATAC-seq processed data and code This study https://doi.org/10.5281/zenodo.8266662 ATAC-seq sequence data This study GEO: GSE241229 Experimental models: Organisms/strains NSG mice (NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ) Jackson Laboratory N/A Recombinant DNA EGFP-VSVg-ProtC-AU1 Addgene N/A mCherry-StrepTagII-FLAG-AU1 Addgene N/A Software and algorithms RStudio RStudio rstudio.com Cytobank BeckmanCoulter beckman.com CellEngine Primity Bio cellengine.com MATLAB MathWorks mathworks.co Cell Reports 44, 115913, July 22, 2025 19

Article Title: Effectorless Fc-fusion improves FLT3L drug-like properties for cancer immunotherapy combinations.
Article Snippet: 41 CD34+ human cord blood stem cells (StemCell Technologies) were seeded at 5000 cells per well in 96-well U-bottom plates (Costar) in 100 μL expansion media (StemSpan media–StemCell Technologies, 10% FBS, 40 ng/mL IL-3, 200 ng/mL SCF, 100 ng/mL TPO–cytokines from Peprotech).

Article Title: Lactate dehydrogenase A-coupled NAD + regeneration is critical for acute myeloid leukemia cell survival
Article Snippet: CD34 + human cord blood-derived cells were obtained from STEMCELL Technologies and were maintained in StemSpan SFEM-II with CD34 supplements (STEMCELL Technologies).

Article Title: Perturbing LSD1 and WNT rewires transcription to synergistically induce AML differentiation.
Article Snippet: Human cord blood CD34+ cells (200-0000, StemCell Technologies) were plated in methylcellulose (MethoCult H4534 Classic, StemCell Technologies).

Article Title: Lactate dehydrogenase A-coupled NAD + regeneration is critical for acute myeloid leukemia cell survival.
Article Snippet: CD34+ human cord blood-derived cells were obtained from STEMCELL Technologies and were maintained in StemSpan SFEM-II with CD34 supplements (STEMCELL Technologies).



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humanized cd34+ hu-nsg mouse model grafted with primary human cd34+ cells isolated from cord blood - by Bioz Stars, 2026-09
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In vitro characterisation of FLT3L-Fc NG2LH activity . Representative sensorgrams of FLT3L-Fc NG2LH binding to recombinant human monomeric FLT3-monoFc (a) and dimeric FLT3-Fc (b). Black solid lines are curves fit to the data using a 1:1 binding model, and coloured lines are raw data. The tested recombinant human FLT3-monoFc concentrations (from bottom to top) are 62.5, 125, 250, 500, 1000, and 2000 nM; recombinant human dimeric FLT3-Fc concentrations (from bottom to top) are 3.75, 7.5, 15, 30, 60, and 120 nM. The sensorgrams were generated after in-line reference cell correction followed by buffer sample subtraction. The experiments were conducted at 37 °C. (c) Binding affinities of recombinant human monomeric FLT3-monoFc and dimeric FLT3-Fc proteins to captured FLT3L-Fc NG2LH. k a association rate constant; k d dissociation rate constant; K D equilibrium dissociation constant. Data for FLT3L-Fc NG2LH binding to recombinant human FLT3-monoFc were averaged from three independent experimental Biacore runs. Data for FLT3L-Fc NG2LH binding to recombinant human dimeric FLT3-Fc were averaged from two independent experimental Biacore runs. (d) ADCP for FLT3L-Fc NG2LH and its variants. Data shown are a representative set taken from one of three independent experiments. Average (SD) of duplicate values are shown. (e) Proliferation of OCI-AML5 cells in vitro. A representative dose response curve is shown, Average (SD) of triplicate values. Data were normalised to 10 μg/mL FLT3L-Fc NG2LH as the maximum (100%) response and the assay media alone control as the minimum (0%) response. (f) Human cDC1 differentiation from cord blood <t>stem</t> <t>cells.</t> Frequency of cDC1 at the end of culture is shown (percent of live cells). Results were obtained from 2 donors. (g) In vitro proliferation of mouse bone marrow cells in response to mFLT3L or mFLT3L-Fc. Average (SD) of duplicate values are shown.
Cd34 + Human Cord Blood Stem Cells, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cord+blood+cd34++cells/4d+nucleofector/pm40645177-875-9-21
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cd34 + human cord blood stem cells - by Bioz Stars, 2026-09
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STEMCELL Technologies Inc cord blood cd34+ cells
In vitro characterisation of FLT3L-Fc NG2LH activity . Representative sensorgrams of FLT3L-Fc NG2LH binding to recombinant human monomeric FLT3-monoFc (a) and dimeric FLT3-Fc (b). Black solid lines are curves fit to the data using a 1:1 binding model, and coloured lines are raw data. The tested recombinant human FLT3-monoFc concentrations (from bottom to top) are 62.5, 125, 250, 500, 1000, and 2000 nM; recombinant human dimeric FLT3-Fc concentrations (from bottom to top) are 3.75, 7.5, 15, 30, 60, and 120 nM. The sensorgrams were generated after in-line reference cell correction followed by buffer sample subtraction. The experiments were conducted at 37 °C. (c) Binding affinities of recombinant human monomeric FLT3-monoFc and dimeric FLT3-Fc proteins to captured FLT3L-Fc NG2LH. k a association rate constant; k d dissociation rate constant; K D equilibrium dissociation constant. Data for FLT3L-Fc NG2LH binding to recombinant human FLT3-monoFc were averaged from three independent experimental Biacore runs. Data for FLT3L-Fc NG2LH binding to recombinant human dimeric FLT3-Fc were averaged from two independent experimental Biacore runs. (d) ADCP for FLT3L-Fc NG2LH and its variants. Data shown are a representative set taken from one of three independent experiments. Average (SD) of duplicate values are shown. (e) Proliferation of OCI-AML5 cells in vitro. A representative dose response curve is shown, Average (SD) of triplicate values. Data were normalised to 10 μg/mL FLT3L-Fc NG2LH as the maximum (100%) response and the assay media alone control as the minimum (0%) response. (f) Human cDC1 differentiation from cord blood <t>stem</t> <t>cells.</t> Frequency of cDC1 at the end of culture is shown (percent of live cells). Results were obtained from 2 donors. (g) In vitro proliferation of mouse bone marrow cells in response to mFLT3L or mFLT3L-Fc. Average (SD) of duplicate values are shown.
Cord Blood Cd34+ Cells, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cord+blood+cd34++cells/easysep+human+t+cell+isolation+kit/pm40577131-246-36-40
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cord blood cd34+ cells - by Bioz Stars, 2026-09
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STEMCELL Technologies Inc human cord blood cd34+ cell
In vitro characterisation of FLT3L-Fc NG2LH activity . Representative sensorgrams of FLT3L-Fc NG2LH binding to recombinant human monomeric FLT3-monoFc (a) and dimeric FLT3-Fc (b). Black solid lines are curves fit to the data using a 1:1 binding model, and coloured lines are raw data. The tested recombinant human FLT3-monoFc concentrations (from bottom to top) are 62.5, 125, 250, 500, 1000, and 2000 nM; recombinant human dimeric FLT3-Fc concentrations (from bottom to top) are 3.75, 7.5, 15, 30, 60, and 120 nM. The sensorgrams were generated after in-line reference cell correction followed by buffer sample subtraction. The experiments were conducted at 37 °C. (c) Binding affinities of recombinant human monomeric FLT3-monoFc and dimeric FLT3-Fc proteins to captured FLT3L-Fc NG2LH. k a association rate constant; k d dissociation rate constant; K D equilibrium dissociation constant. Data for FLT3L-Fc NG2LH binding to recombinant human FLT3-monoFc were averaged from three independent experimental Biacore runs. Data for FLT3L-Fc NG2LH binding to recombinant human dimeric FLT3-Fc were averaged from two independent experimental Biacore runs. (d) ADCP for FLT3L-Fc NG2LH and its variants. Data shown are a representative set taken from one of three independent experiments. Average (SD) of duplicate values are shown. (e) Proliferation of OCI-AML5 cells in vitro. A representative dose response curve is shown, Average (SD) of triplicate values. Data were normalised to 10 μg/mL FLT3L-Fc NG2LH as the maximum (100%) response and the assay media alone control as the minimum (0%) response. (f) Human cDC1 differentiation from cord blood <t>stem</t> <t>cells.</t> Frequency of cDC1 at the end of culture is shown (percent of live cells). Results were obtained from 2 donors. (g) In vitro proliferation of mouse bone marrow cells in response to mFLT3L or mFLT3L-Fc. Average (SD) of duplicate values are shown.
Human Cord Blood Cd34+ Cell, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cord+blood+cd34++cells/easysep+human+t+cell+isolation+kit/pmc12261347-80-0-12
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Charles River Laboratories human cord blood cd34 + hematopoietic stem and progenitor cells (hspcs)
In vitro characterisation of FLT3L-Fc NG2LH activity . Representative sensorgrams of FLT3L-Fc NG2LH binding to recombinant human monomeric FLT3-monoFc (a) and dimeric FLT3-Fc (b). Black solid lines are curves fit to the data using a 1:1 binding model, and coloured lines are raw data. The tested recombinant human FLT3-monoFc concentrations (from bottom to top) are 62.5, 125, 250, 500, 1000, and 2000 nM; recombinant human dimeric FLT3-Fc concentrations (from bottom to top) are 3.75, 7.5, 15, 30, 60, and 120 nM. The sensorgrams were generated after in-line reference cell correction followed by buffer sample subtraction. The experiments were conducted at 37 °C. (c) Binding affinities of recombinant human monomeric FLT3-monoFc and dimeric FLT3-Fc proteins to captured FLT3L-Fc NG2LH. k a association rate constant; k d dissociation rate constant; K D equilibrium dissociation constant. Data for FLT3L-Fc NG2LH binding to recombinant human FLT3-monoFc were averaged from three independent experimental Biacore runs. Data for FLT3L-Fc NG2LH binding to recombinant human dimeric FLT3-Fc were averaged from two independent experimental Biacore runs. (d) ADCP for FLT3L-Fc NG2LH and its variants. Data shown are a representative set taken from one of three independent experiments. Average (SD) of duplicate values are shown. (e) Proliferation of OCI-AML5 cells in vitro. A representative dose response curve is shown, Average (SD) of triplicate values. Data were normalised to 10 μg/mL FLT3L-Fc NG2LH as the maximum (100%) response and the assay media alone control as the minimum (0%) response. (f) Human cDC1 differentiation from cord blood <t>stem</t> <t>cells.</t> Frequency of cDC1 at the end of culture is shown (percent of live cells). Results were obtained from 2 donors. (g) In vitro proliferation of mouse bone marrow cells in response to mFLT3L or mFLT3L-Fc. Average (SD) of duplicate values are shown.
Human Cord Blood Cd34 + Hematopoietic Stem And Progenitor Cells (Hspcs), supplied by Charles River Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cord+blood+cd34++cells/cd34++cells/pmc12090235-56-0-12
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Tregs are depleted by the CCR4-CAR in a humanized mouse model. (A) Experimental design. NSG-SGM3-IL15 engrafted with CD34 + hematopoietic stem cells were injected with 1 million CAR + CCR4-CARTs IV. Blood was collected on days 0, 3, 5, and 8, and mice were euthanized on day 11. (B) Representative flow plots showing the proportion of human CD45 (hCD45) and mCD45 leukocytes at baseline. (C) Proportion of Tregs, CD4 + non-Treg, and CD4 − cells of hCD45 percent at baseline. (D) Percentage of Tregs, non-Treg, and CD4 − cells that are CCR4 + at baseline. (E) Representative flow plots showing the CCR4 + and FOXP3 + expression on the CD4 + population before and after CART administration gated on CD4 + cells. (F-K) Proportions of Tregs, CD4 + non-Tregs, and CD4 − cells over time. Significance was determined using t tests corrected for multiple comparisons, with comparison to baseline indicated on graph; ∗ P < .05; ∗∗ P < .01; ∗∗∗ P < .001; ∗∗∗∗ P < .0001. M1, Mouse 1; M2, Mouse 2; M3, Mouse 3; mCD45, mouse CD45.

Journal: Blood Advances

Article Title: CAR T cells targeting CCR4 selectively deplete human Tregs ex vivo and in vivo

doi: 10.1182/bloodadvances.2025017573

Figure Lengend Snippet: Tregs are depleted by the CCR4-CAR in a humanized mouse model. (A) Experimental design. NSG-SGM3-IL15 engrafted with CD34 + hematopoietic stem cells were injected with 1 million CAR + CCR4-CARTs IV. Blood was collected on days 0, 3, 5, and 8, and mice were euthanized on day 11. (B) Representative flow plots showing the proportion of human CD45 (hCD45) and mCD45 leukocytes at baseline. (C) Proportion of Tregs, CD4 + non-Treg, and CD4 − cells of hCD45 percent at baseline. (D) Percentage of Tregs, non-Treg, and CD4 − cells that are CCR4 + at baseline. (E) Representative flow plots showing the CCR4 + and FOXP3 + expression on the CD4 + population before and after CART administration gated on CD4 + cells. (F-K) Proportions of Tregs, CD4 + non-Tregs, and CD4 − cells over time. Significance was determined using t tests corrected for multiple comparisons, with comparison to baseline indicated on graph; ∗ P < .05; ∗∗ P < .01; ∗∗∗ P < .001; ∗∗∗∗ P < .0001. M1, Mouse 1; M2, Mouse 2; M3, Mouse 3; mCD45, mouse CD45.

Article Snippet: Mice were engrafted with human cord blood–derived CD34 + hematopoietic stem cells, and females were available for use at age 13 to 18 weeks after evaluation of engrafted human cell populations by flow cytometry at The Jackson Laboratory.

Techniques: Injection, Expressing, Comparison

In vitro characterisation of FLT3L-Fc NG2LH activity . Representative sensorgrams of FLT3L-Fc NG2LH binding to recombinant human monomeric FLT3-monoFc (a) and dimeric FLT3-Fc (b). Black solid lines are curves fit to the data using a 1:1 binding model, and coloured lines are raw data. The tested recombinant human FLT3-monoFc concentrations (from bottom to top) are 62.5, 125, 250, 500, 1000, and 2000 nM; recombinant human dimeric FLT3-Fc concentrations (from bottom to top) are 3.75, 7.5, 15, 30, 60, and 120 nM. The sensorgrams were generated after in-line reference cell correction followed by buffer sample subtraction. The experiments were conducted at 37 °C. (c) Binding affinities of recombinant human monomeric FLT3-monoFc and dimeric FLT3-Fc proteins to captured FLT3L-Fc NG2LH. k a association rate constant; k d dissociation rate constant; K D equilibrium dissociation constant. Data for FLT3L-Fc NG2LH binding to recombinant human FLT3-monoFc were averaged from three independent experimental Biacore runs. Data for FLT3L-Fc NG2LH binding to recombinant human dimeric FLT3-Fc were averaged from two independent experimental Biacore runs. (d) ADCP for FLT3L-Fc NG2LH and its variants. Data shown are a representative set taken from one of three independent experiments. Average (SD) of duplicate values are shown. (e) Proliferation of OCI-AML5 cells in vitro. A representative dose response curve is shown, Average (SD) of triplicate values. Data were normalised to 10 μg/mL FLT3L-Fc NG2LH as the maximum (100%) response and the assay media alone control as the minimum (0%) response. (f) Human cDC1 differentiation from cord blood stem cells. Frequency of cDC1 at the end of culture is shown (percent of live cells). Results were obtained from 2 donors. (g) In vitro proliferation of mouse bone marrow cells in response to mFLT3L or mFLT3L-Fc. Average (SD) of duplicate values are shown.

Journal: eBioMedicine

Article Title: Effectorless Fc-fusion improves FLT3L drug-like properties for cancer immunotherapy combinations

doi: 10.1016/j.ebiom.2025.105822

Figure Lengend Snippet: In vitro characterisation of FLT3L-Fc NG2LH activity . Representative sensorgrams of FLT3L-Fc NG2LH binding to recombinant human monomeric FLT3-monoFc (a) and dimeric FLT3-Fc (b). Black solid lines are curves fit to the data using a 1:1 binding model, and coloured lines are raw data. The tested recombinant human FLT3-monoFc concentrations (from bottom to top) are 62.5, 125, 250, 500, 1000, and 2000 nM; recombinant human dimeric FLT3-Fc concentrations (from bottom to top) are 3.75, 7.5, 15, 30, 60, and 120 nM. The sensorgrams were generated after in-line reference cell correction followed by buffer sample subtraction. The experiments were conducted at 37 °C. (c) Binding affinities of recombinant human monomeric FLT3-monoFc and dimeric FLT3-Fc proteins to captured FLT3L-Fc NG2LH. k a association rate constant; k d dissociation rate constant; K D equilibrium dissociation constant. Data for FLT3L-Fc NG2LH binding to recombinant human FLT3-monoFc were averaged from three independent experimental Biacore runs. Data for FLT3L-Fc NG2LH binding to recombinant human dimeric FLT3-Fc were averaged from two independent experimental Biacore runs. (d) ADCP for FLT3L-Fc NG2LH and its variants. Data shown are a representative set taken from one of three independent experiments. Average (SD) of duplicate values are shown. (e) Proliferation of OCI-AML5 cells in vitro. A representative dose response curve is shown, Average (SD) of triplicate values. Data were normalised to 10 μg/mL FLT3L-Fc NG2LH as the maximum (100%) response and the assay media alone control as the minimum (0%) response. (f) Human cDC1 differentiation from cord blood stem cells. Frequency of cDC1 at the end of culture is shown (percent of live cells). Results were obtained from 2 donors. (g) In vitro proliferation of mouse bone marrow cells in response to mFLT3L or mFLT3L-Fc. Average (SD) of duplicate values are shown.

Article Snippet: CD34+ human cord blood stem cells (StemCell Technologies) were seeded at 5000 cells per well in 96-well U-bottom plates (Costar) in 100 μL expansion media (StemSpan media–StemCell Technologies, 10% FBS, 40 ng/mL IL-3, 200 ng/mL SCF, 100 ng/mL TPO–cytokines from Peprotech).

Techniques: In Vitro, Activity Assay, Binding Assay, Recombinant, Generated, Control