cd34+ human cord blood stem cells (STEMCELL Technologies Inc)
Structured Review

Cd34+ Human Cord Blood Stem Cells, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cord+blood+cd34++cells/easysep+human+t+cell+isolation+kit/pmc12272481-113-0-6
Average 90 stars, based on 1 article reviews
Images
1) Product Images from "Effectorless Fc-fusion improves FLT3L drug-like properties for cancer immunotherapy combinations"
Article Title: Effectorless Fc-fusion improves FLT3L drug-like properties for cancer immunotherapy combinations
Journal: eBioMedicine
doi: 10.1016/j.ebiom.2025.105822
Figure Legend Snippet: In vitro characterisation of FLT3L-Fc NG2LH activity . Representative sensorgrams of FLT3L-Fc NG2LH binding to recombinant human monomeric FLT3-monoFc (a) and dimeric FLT3-Fc (b). Black solid lines are curves fit to the data using a 1:1 binding model, and coloured lines are raw data. The tested recombinant human FLT3-monoFc concentrations (from bottom to top) are 62.5, 125, 250, 500, 1000, and 2000 nM; recombinant human dimeric FLT3-Fc concentrations (from bottom to top) are 3.75, 7.5, 15, 30, 60, and 120 nM. The sensorgrams were generated after in-line reference cell correction followed by buffer sample subtraction. The experiments were conducted at 37 °C. (c) Binding affinities of recombinant human monomeric FLT3-monoFc and dimeric FLT3-Fc proteins to captured FLT3L-Fc NG2LH. k a association rate constant; k d dissociation rate constant; K D equilibrium dissociation constant. Data for FLT3L-Fc NG2LH binding to recombinant human FLT3-monoFc were averaged from three independent experimental Biacore runs. Data for FLT3L-Fc NG2LH binding to recombinant human dimeric FLT3-Fc were averaged from two independent experimental Biacore runs. (d) ADCP for FLT3L-Fc NG2LH and its variants. Data shown are a representative set taken from one of three independent experiments. Average (SD) of duplicate values are shown. (e) Proliferation of OCI-AML5 cells in vitro. A representative dose response curve is shown, Average (SD) of triplicate values. Data were normalised to 10 μg/mL FLT3L-Fc NG2LH as the maximum (100%) response and the assay media alone control as the minimum (0%) response. (f) Human cDC1 differentiation from cord blood stem cells. Frequency of cDC1 at the end of culture is shown (percent of live cells). Results were obtained from 2 donors. (g) In vitro proliferation of mouse bone marrow cells in response to mFLT3L or mFLT3L-Fc. Average (SD) of duplicate values are shown.
Techniques Used: In Vitro, Activity Assay, Binding Assay, Recombinant, Generated, Control
Related Articles
In Vitro:Article Title: Effectorless Fc-fusion improves FLT3L drug-like properties for cancer immunotherapy combinations Article Snippet: Article Title: Perturbing LSD1 and WNT rewires transcription to synergistically induce AML differentiation Article Snippet: Article Title: Utility of CK8/18 in identifying circulating tumor cells derived from lesions in patients with non-small cell lung cancer Article Snippet: Article Title: Quantification of intrinsic regulatory factors refines human hematopoietic progenitor definitions and reveals early erythroid lineage priming. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies All antibodies used for cytometry Various Table S1 Article Title: Effectorless Fc-fusion improves FLT3L drug-like properties for cancer immunotherapy combinations. Article Snippet: 41 Article Title: Lactate dehydrogenase A-coupled NAD + regeneration is critical for acute myeloid leukemia cell survival Article Snippet: Article Title: Perturbing LSD1 and WNT rewires transcription to synergistically induce AML differentiation. Article Snippet: Article Title: Lactate dehydrogenase A-coupled NAD + regeneration is critical for acute myeloid leukemia cell survival. Article Snippet: Activity Assay:Article Title: Effectorless Fc-fusion improves FLT3L drug-like properties for cancer immunotherapy combinations Article Snippet: Article Title: Perturbing LSD1 and WNT rewires transcription to synergistically induce AML differentiation Article Snippet: Article Title: Utility of CK8/18 in identifying circulating tumor cells derived from lesions in patients with non-small cell lung cancer Article Snippet: Article Title: Quantification of intrinsic regulatory factors refines human hematopoietic progenitor definitions and reveals early erythroid lineage priming. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies All antibodies used for cytometry Various Table S1 Article Title: Effectorless Fc-fusion improves FLT3L drug-like properties for cancer immunotherapy combinations. Article Snippet: 41 Article Title: Lactate dehydrogenase A-coupled NAD + regeneration is critical for acute myeloid leukemia cell survival Article Snippet: Article Title: Perturbing LSD1 and WNT rewires transcription to synergistically induce AML differentiation. Article Snippet: Article Title: Lactate dehydrogenase A-coupled NAD + regeneration is critical for acute myeloid leukemia cell survival. Article Snippet: Binding Assay:Article Title: Effectorless Fc-fusion improves FLT3L drug-like properties for cancer immunotherapy combinations Article Snippet: Article Title: Perturbing LSD1 and WNT rewires transcription to synergistically induce AML differentiation Article Snippet: Article Title: Utility of CK8/18 in identifying circulating tumor cells derived from lesions in patients with non-small cell lung cancer Article Snippet: Article Title: Quantification of intrinsic regulatory factors refines human hematopoietic progenitor definitions and reveals early erythroid lineage priming. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies All antibodies used for cytometry Various Table S1 Article Title: Effectorless Fc-fusion improves FLT3L drug-like properties for cancer immunotherapy combinations. Article Snippet: 41 Article Title: Lactate dehydrogenase A-coupled NAD + regeneration is critical for acute myeloid leukemia cell survival Article Snippet: Article Title: Perturbing LSD1 and WNT rewires transcription to synergistically induce AML differentiation. Article Snippet: Article Title: Lactate dehydrogenase A-coupled NAD + regeneration is critical for acute myeloid leukemia cell survival. Article Snippet: Recombinant:Article Title: Effectorless Fc-fusion improves FLT3L drug-like properties for cancer immunotherapy combinations Article Snippet: Article Title: Perturbing LSD1 and WNT rewires transcription to synergistically induce AML differentiation Article Snippet: Article Title: Utility of CK8/18 in identifying circulating tumor cells derived from lesions in patients with non-small cell lung cancer Article Snippet: Article Title: Quantification of intrinsic regulatory factors refines human hematopoietic progenitor definitions and reveals early erythroid lineage priming. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies All antibodies used for cytometry Various Table S1 Article Title: Effectorless Fc-fusion improves FLT3L drug-like properties for cancer immunotherapy combinations. Article Snippet: 41 Article Title: Lactate dehydrogenase A-coupled NAD + regeneration is critical for acute myeloid leukemia cell survival Article Snippet: Article Title: Perturbing LSD1 and WNT rewires transcription to synergistically induce AML differentiation. Article Snippet: Article Title: Lactate dehydrogenase A-coupled NAD + regeneration is critical for acute myeloid leukemia cell survival. Article Snippet: Generated:Article Title: Effectorless Fc-fusion improves FLT3L drug-like properties for cancer immunotherapy combinations Article Snippet: Article Title: Perturbing LSD1 and WNT rewires transcription to synergistically induce AML differentiation Article Snippet: Article Title: Utility of CK8/18 in identifying circulating tumor cells derived from lesions in patients with non-small cell lung cancer Article Snippet: Article Title: Quantification of intrinsic regulatory factors refines human hematopoietic progenitor definitions and reveals early erythroid lineage priming. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies All antibodies used for cytometry Various Table S1 Article Title: Effectorless Fc-fusion improves FLT3L drug-like properties for cancer immunotherapy combinations. Article Snippet: 41 Article Title: Lactate dehydrogenase A-coupled NAD + regeneration is critical for acute myeloid leukemia cell survival Article Snippet: Article Title: Perturbing LSD1 and WNT rewires transcription to synergistically induce AML differentiation. Article Snippet: Article Title: Lactate dehydrogenase A-coupled NAD + regeneration is critical for acute myeloid leukemia cell survival. Article Snippet: Control:Article Title: Effectorless Fc-fusion improves FLT3L drug-like properties for cancer immunotherapy combinations Article Snippet: Article Title: Perturbing LSD1 and WNT rewires transcription to synergistically induce AML differentiation Article Snippet: Article Title: Utility of CK8/18 in identifying circulating tumor cells derived from lesions in patients with non-small cell lung cancer Article Snippet: Article Title: Quantification of intrinsic regulatory factors refines human hematopoietic progenitor definitions and reveals early erythroid lineage priming. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies All antibodies used for cytometry Various Table S1 Article Title: Effectorless Fc-fusion improves FLT3L drug-like properties for cancer immunotherapy combinations. Article Snippet: 41 Article Title: Lactate dehydrogenase A-coupled NAD + regeneration is critical for acute myeloid leukemia cell survival Article Snippet: Article Title: Perturbing LSD1 and WNT rewires transcription to synergistically induce AML differentiation. Article Snippet: Article Title: Lactate dehydrogenase A-coupled NAD + regeneration is critical for acute myeloid leukemia cell survival. Article Snippet: Cytometry:Article Title: Effectorless Fc-fusion improves FLT3L drug-like properties for cancer immunotherapy combinations Article Snippet: Article Title: Perturbing LSD1 and WNT rewires transcription to synergistically induce AML differentiation Article Snippet: Article Title: Utility of CK8/18 in identifying circulating tumor cells derived from lesions in patients with non-small cell lung cancer Article Snippet: Article Title: Quantification of intrinsic regulatory factors refines human hematopoietic progenitor definitions and reveals early erythroid lineage priming. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies All antibodies used for cytometry Various Table S1 Article Title: Effectorless Fc-fusion improves FLT3L drug-like properties for cancer immunotherapy combinations. Article Snippet: 41 Article Title: Lactate dehydrogenase A-coupled NAD + regeneration is critical for acute myeloid leukemia cell survival Article Snippet: Article Title: Perturbing LSD1 and WNT rewires transcription to synergistically induce AML differentiation. Article Snippet: Article Title: Lactate dehydrogenase A-coupled NAD + regeneration is critical for acute myeloid leukemia cell survival. Article Snippet: Blocking Assay:Article Title: Effectorless Fc-fusion improves FLT3L drug-like properties for cancer immunotherapy combinations Article Snippet: Article Title: Perturbing LSD1 and WNT rewires transcription to synergistically induce AML differentiation Article Snippet: Article Title: Utility of CK8/18 in identifying circulating tumor cells derived from lesions in patients with non-small cell lung cancer Article Snippet: Article Title: Quantification of intrinsic regulatory factors refines human hematopoietic progenitor definitions and reveals early erythroid lineage priming. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies All antibodies used for cytometry Various Table S1 Article Title: Effectorless Fc-fusion improves FLT3L drug-like properties for cancer immunotherapy combinations. Article Snippet: 41 Article Title: Lactate dehydrogenase A-coupled NAD + regeneration is critical for acute myeloid leukemia cell survival Article Snippet: Article Title: Perturbing LSD1 and WNT rewires transcription to synergistically induce AML differentiation. Article Snippet: Article Title: Lactate dehydrogenase A-coupled NAD + regeneration is critical for acute myeloid leukemia cell survival. Article Snippet: Staining:Article Title: Effectorless Fc-fusion improves FLT3L drug-like properties for cancer immunotherapy combinations Article Snippet: Article Title: Perturbing LSD1 and WNT rewires transcription to synergistically induce AML differentiation Article Snippet: Article Title: Utility of CK8/18 in identifying circulating tumor cells derived from lesions in patients with non-small cell lung cancer Article Snippet: Article Title: Quantification of intrinsic regulatory factors refines human hematopoietic progenitor definitions and reveals early erythroid lineage priming. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies All antibodies used for cytometry Various Table S1 Article Title: Effectorless Fc-fusion improves FLT3L drug-like properties for cancer immunotherapy combinations. Article Snippet: 41 Article Title: Lactate dehydrogenase A-coupled NAD + regeneration is critical for acute myeloid leukemia cell survival Article Snippet: Article Title: Perturbing LSD1 and WNT rewires transcription to synergistically induce AML differentiation. Article Snippet: Article Title: Lactate dehydrogenase A-coupled NAD + regeneration is critical for acute myeloid leukemia cell survival. Article Snippet: Mass Cytometry:Article Title: Effectorless Fc-fusion improves FLT3L drug-like properties for cancer immunotherapy combinations Article Snippet: Article Title: Perturbing LSD1 and WNT rewires transcription to synergistically induce AML differentiation Article Snippet: Article Title: Utility of CK8/18 in identifying circulating tumor cells derived from lesions in patients with non-small cell lung cancer Article Snippet: Article Title: Quantification of intrinsic regulatory factors refines human hematopoietic progenitor definitions and reveals early erythroid lineage priming. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies All antibodies used for cytometry Various Table S1 Article Title: Effectorless Fc-fusion improves FLT3L drug-like properties for cancer immunotherapy combinations. Article Snippet: 41 Article Title: Lactate dehydrogenase A-coupled NAD + regeneration is critical for acute myeloid leukemia cell survival Article Snippet: Article Title: Perturbing LSD1 and WNT rewires transcription to synergistically induce AML differentiation. Article Snippet: Article Title: Lactate dehydrogenase A-coupled NAD + regeneration is critical for acute myeloid leukemia cell survival. Article Snippet: Sequencing:Article Title: Effectorless Fc-fusion improves FLT3L drug-like properties for cancer immunotherapy combinations Article Snippet: Article Title: Perturbing LSD1 and WNT rewires transcription to synergistically induce AML differentiation Article Snippet: Article Title: Utility of CK8/18 in identifying circulating tumor cells derived from lesions in patients with non-small cell lung cancer Article Snippet: Article Title: Quantification of intrinsic regulatory factors refines human hematopoietic progenitor definitions and reveals early erythroid lineage priming. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies All antibodies used for cytometry Various Table S1 Article Title: Effectorless Fc-fusion improves FLT3L drug-like properties for cancer immunotherapy combinations. Article Snippet: 41 Article Title: Lactate dehydrogenase A-coupled NAD + regeneration is critical for acute myeloid leukemia cell survival Article Snippet: Article Title: Perturbing LSD1 and WNT rewires transcription to synergistically induce AML differentiation. Article Snippet: Article Title: Lactate dehydrogenase A-coupled NAD + regeneration is critical for acute myeloid leukemia cell survival. Article Snippet: Software:Article Title: Effectorless Fc-fusion improves FLT3L drug-like properties for cancer immunotherapy combinations Article Snippet: Article Title: Perturbing LSD1 and WNT rewires transcription to synergistically induce AML differentiation Article Snippet: Article Title: Utility of CK8/18 in identifying circulating tumor cells derived from lesions in patients with non-small cell lung cancer Article Snippet: Article Title: Quantification of intrinsic regulatory factors refines human hematopoietic progenitor definitions and reveals early erythroid lineage priming. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies All antibodies used for cytometry Various Table S1 Article Title: Effectorless Fc-fusion improves FLT3L drug-like properties for cancer immunotherapy combinations. Article Snippet: 41 Article Title: Lactate dehydrogenase A-coupled NAD + regeneration is critical for acute myeloid leukemia cell survival Article Snippet: Article Title: Perturbing LSD1 and WNT rewires transcription to synergistically induce AML differentiation. Article Snippet: Article Title: Lactate dehydrogenase A-coupled NAD + regeneration is critical for acute myeloid leukemia cell survival. Article Snippet: RNA Sequencing:Article Title: Effectorless Fc-fusion improves FLT3L drug-like properties for cancer immunotherapy combinations Article Snippet: Article Title: Perturbing LSD1 and WNT rewires transcription to synergistically induce AML differentiation Article Snippet: Article Title: Utility of CK8/18 in identifying circulating tumor cells derived from lesions in patients with non-small cell lung cancer Article Snippet: Article Title: Quantification of intrinsic regulatory factors refines human hematopoietic progenitor definitions and reveals early erythroid lineage priming. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies All antibodies used for cytometry Various Table S1 Article Title: Effectorless Fc-fusion improves FLT3L drug-like properties for cancer immunotherapy combinations. Article Snippet: 41 Article Title: Lactate dehydrogenase A-coupled NAD + regeneration is critical for acute myeloid leukemia cell survival Article Snippet: Article Title: Perturbing LSD1 and WNT rewires transcription to synergistically induce AML differentiation. Article Snippet: Article Title: Lactate dehydrogenase A-coupled NAD + regeneration is critical for acute myeloid leukemia cell survival. Article Snippet: Western Blot:Article Title: Effectorless Fc-fusion improves FLT3L drug-like properties for cancer immunotherapy combinations Article Snippet: Article Title: Perturbing LSD1 and WNT rewires transcription to synergistically induce AML differentiation Article Snippet: Article Title: Utility of CK8/18 in identifying circulating tumor cells derived from lesions in patients with non-small cell lung cancer Article Snippet: Article Title: Quantification of intrinsic regulatory factors refines human hematopoietic progenitor definitions and reveals early erythroid lineage priming. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies All antibodies used for cytometry Various Table S1 Article Title: Effectorless Fc-fusion improves FLT3L drug-like properties for cancer immunotherapy combinations. Article Snippet: 41 Article Title: Lactate dehydrogenase A-coupled NAD + regeneration is critical for acute myeloid leukemia cell survival Article Snippet: Article Title: Perturbing LSD1 and WNT rewires transcription to synergistically induce AML differentiation. Article Snippet: Article Title: Lactate dehydrogenase A-coupled NAD + regeneration is critical for acute myeloid leukemia cell survival. Article Snippet: Expressing:Article Title: Effectorless Fc-fusion improves FLT3L drug-like properties for cancer immunotherapy combinations Article Snippet: Article Title: Perturbing LSD1 and WNT rewires transcription to synergistically induce AML differentiation Article Snippet: Article Title: Utility of CK8/18 in identifying circulating tumor cells derived from lesions in patients with non-small cell lung cancer Article Snippet: Article Title: Quantification of intrinsic regulatory factors refines human hematopoietic progenitor definitions and reveals early erythroid lineage priming. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies All antibodies used for cytometry Various Table S1 Article Title: Effectorless Fc-fusion improves FLT3L drug-like properties for cancer immunotherapy combinations. Article Snippet: 41 Article Title: Lactate dehydrogenase A-coupled NAD + regeneration is critical for acute myeloid leukemia cell survival Article Snippet: Article Title: Perturbing LSD1 and WNT rewires transcription to synergistically induce AML differentiation. Article Snippet: Article Title: Lactate dehydrogenase A-coupled NAD + regeneration is critical for acute myeloid leukemia cell survival. Article Snippet: Two Tailed Test:Article Title: Effectorless Fc-fusion improves FLT3L drug-like properties for cancer immunotherapy combinations Article Snippet: Article Title: Perturbing LSD1 and WNT rewires transcription to synergistically induce AML differentiation Article Snippet: Article Title: Utility of CK8/18 in identifying circulating tumor cells derived from lesions in patients with non-small cell lung cancer Article Snippet: Article Title: Quantification of intrinsic regulatory factors refines human hematopoietic progenitor definitions and reveals early erythroid lineage priming. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies All antibodies used for cytometry Various Table S1 Article Title: Effectorless Fc-fusion improves FLT3L drug-like properties for cancer immunotherapy combinations. Article Snippet: 41 Article Title: Lactate dehydrogenase A-coupled NAD + regeneration is critical for acute myeloid leukemia cell survival Article Snippet: Article Title: Perturbing LSD1 and WNT rewires transcription to synergistically induce AML differentiation. Article Snippet: Article Title: Lactate dehydrogenase A-coupled NAD + regeneration is critical for acute myeloid leukemia cell survival. Article Snippet: Dot Blot:Article Title: Effectorless Fc-fusion improves FLT3L drug-like properties for cancer immunotherapy combinations Article Snippet: Article Title: Perturbing LSD1 and WNT rewires transcription to synergistically induce AML differentiation Article Snippet: Article Title: Utility of CK8/18 in identifying circulating tumor cells derived from lesions in patients with non-small cell lung cancer Article Snippet: Article Title: Quantification of intrinsic regulatory factors refines human hematopoietic progenitor definitions and reveals early erythroid lineage priming. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies All antibodies used for cytometry Various Table S1 Article Title: Effectorless Fc-fusion improves FLT3L drug-like properties for cancer immunotherapy combinations. Article Snippet: 41 Article Title: Lactate dehydrogenase A-coupled NAD + regeneration is critical for acute myeloid leukemia cell survival Article Snippet: Article Title: Perturbing LSD1 and WNT rewires transcription to synergistically induce AML differentiation. Article Snippet: Article Title: Lactate dehydrogenase A-coupled NAD + regeneration is critical for acute myeloid leukemia cell survival. Article Snippet: Cell Culture:Article Title: Effectorless Fc-fusion improves FLT3L drug-like properties for cancer immunotherapy combinations Article Snippet: Article Title: Perturbing LSD1 and WNT rewires transcription to synergistically induce AML differentiation Article Snippet: Article Title: Utility of CK8/18 in identifying circulating tumor cells derived from lesions in patients with non-small cell lung cancer Article Snippet: Article Title: Quantification of intrinsic regulatory factors refines human hematopoietic progenitor definitions and reveals early erythroid lineage priming. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies All antibodies used for cytometry Various Table S1 Article Title: Effectorless Fc-fusion improves FLT3L drug-like properties for cancer immunotherapy combinations. Article Snippet: 41 Article Title: Lactate dehydrogenase A-coupled NAD + regeneration is critical for acute myeloid leukemia cell survival Article Snippet: Article Title: Perturbing LSD1 and WNT rewires transcription to synergistically induce AML differentiation. Article Snippet: Article Title: Lactate dehydrogenase A-coupled NAD + regeneration is critical for acute myeloid leukemia cell survival. Article Snippet: Mutagenesis:Article Title: Effectorless Fc-fusion improves FLT3L drug-like properties for cancer immunotherapy combinations Article Snippet: Article Title: Perturbing LSD1 and WNT rewires transcription to synergistically induce AML differentiation Article Snippet: Article Title: Utility of CK8/18 in identifying circulating tumor cells derived from lesions in patients with non-small cell lung cancer Article Snippet: Article Title: Quantification of intrinsic regulatory factors refines human hematopoietic progenitor definitions and reveals early erythroid lineage priming. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies All antibodies used for cytometry Various Table S1 Article Title: Effectorless Fc-fusion improves FLT3L drug-like properties for cancer immunotherapy combinations. Article Snippet: 41 Article Title: Lactate dehydrogenase A-coupled NAD + regeneration is critical for acute myeloid leukemia cell survival Article Snippet: Article Title: Perturbing LSD1 and WNT rewires transcription to synergistically induce AML differentiation. Article Snippet: Article Title: Lactate dehydrogenase A-coupled NAD + regeneration is critical for acute myeloid leukemia cell survival. Article Snippet: |
